Rodríguez-González Raquel, Delgado José Ángel, Delgado Luis M, Pérez Román A
Bioengineering Institute of Technology (BIT), Universitat Internacional de Catalunya (UIC), Barcelona, 08017, Spain.
Basic Sciences Department, Faculty of Medicine and Health Sciences, Universitat Internacional de Catalunya, Barcelona, Spain.
Mater Today Bio. 2024 Aug 9;28:101187. doi: 10.1016/j.mtbio.2024.101187. eCollection 2024 Oct.
Silica-based scaffolds are promising in Tissue Engineering by enabling personalized scaffolds, boosting exceptional bioactivity and osteogenic characteristics. Moreover, silica materials are highly tunable, allowing for controlled drug release to enhance tissue regeneration. In this study, we developed a 3D printable silica material with controlled mesoporosity, achieved through the sol-gel reaction of tetraethyl orthosilicate (TEOS) at mild temperatures with the addition of different calcium concentrations. The resultant silica inks exhibited high printability and shape fidelity, while maintaining bioactivity and biocompatibility. Notably, the increased mesopore size enhanced the incorporation and release of large molecules, using cytochrome C as a drug model. Due to the varying surface charge of silica depending on the pH, a pH-dependent control release was obtained between pH 2.5 and 7.5, with maximum release in acidic conditions. Therefore, silica with controlled mesoporosity could be 3D printed, acting as a pH stimuli responsive platform with therapeutic potential.
基于二氧化硅的支架在组织工程领域颇具前景，它能够实现个性化支架，增强卓越的生物活性和成骨特性。此外，二氧化硅材料具有高度可调节性，可实现可控药物释放以促进组织再生。在本研究中，我们通过在温和温度下使正硅酸四乙酯（TEOS）进行溶胶 - 凝胶反应并添加不同钙浓度，开发出一种具有可控介孔率的3D可打印二氧化硅材料。所得的二氧化硅油墨表现出高可打印性和形状保真度，同时保持生物活性和生物相容性。值得注意的是，以细胞色素C作为药物模型，增加的介孔尺寸增强了大分子的掺入和释放。由于二氧化硅的表面电荷随pH值变化，在pH 2.5至7.5之间实现了pH依赖性控释，在酸性条件下释放量最大。因此，具有可控介孔率的二氧化硅可以进行3D打印，作为具有治疗潜力的pH刺激响应平台。
