Kiran Kanchankoti, Rudresh Shoorashetty Manohar, Raksha Yoganand, Ragunatha Shivanna, Jyoti Gupta, Shivaprakash Mandya Rudramurthy
Department of Microbiology, Venereology and Leprosy, ESIC MC PGIMSR and MH, Rajajinagar, Bengaluru, Karnataka, India.
Department of Dermatology, Venereology and Leprosy, ESIC MC PGIMSR and MH, Rajajinagar, Bengaluru, Karnataka, India.
Indian J Pathol Microbiol. 2025 Apr 1;68(2):354-359. doi: 10.4103/ijpm.ijpm_85_24. Epub 2024 Sep 2.
Pityriasis versicolor (PV) is caused by a lipophilic fungus belonging to the genus Malassezia . Potassium hydroxide (KOH) mount is most frequently used for screening of cases and culture is the gold standard. KOH lacks sensitivity, and culture is time-consuming and technically demanding.
AIM, SETTINGS, AND DESIGN: A cross-sectional study was conducted at a tertiary care teaching institution. We aimed to use multiplex-PCR for faster and accurate identification of Malassezia spp directly from skin scrapings of suspected cases of PV.
The study was conducted on suspected cases of PV over a period of 12 months. The clinical and demographic details were recorded. The skin scrapings were subjected to KOH mount and cultured on Sabouraud's dextrose agar with an olive oil overlay. Multiplex-PCR targeting 11 Malassezia spp was performed on DNA extracted from skin scrapings.
A total of 69 suspected cases of PV were studied. Most patients belonged to metro cities and worked in hot and humid climates. The mean duration of lesions was 18 months, and most had macular and patchy lesions. The sensitivity of KOH and culture was found to be 82.6% and 91.3%, respectively. M. globosa (n = 60, 87%) and M. restricta (n = 3, 4.3%) were isolated in culture. Multiplex PCR detected 85.5% of M. globosa , 5.8% of M. restricta , and 8.7% of mixed infection with M. globosa and M. restricta . M-PCR detected Malassezia in all the samples.
M-PCR could identify Malassezia species directly from skin specimens, eliminating the need for culture. M-PCR was accurate, dependable, and exhibited a rapid turnaround time.
花斑糠疹（PV）由马拉色菌属亲脂性真菌引起。氢氧化钾（KOH）涂片是最常用于筛查病例的方法，而培养是金标准。KOH缺乏敏感性，且培养耗时且技术要求高。
目的、地点和设计：在一家三级护理教学机构进行了一项横断面研究。我们旨在使用多重聚合酶链反应（multiplex-PCR）直接从疑似PV病例的皮肤刮屑中更快、更准确地鉴定马拉色菌属菌种。
对疑似PV病例进行了为期12个月的研究。记录了临床和人口统计学细节。对皮肤刮屑进行KOH涂片，并在含橄榄油覆盖层的沙氏葡萄糖琼脂上培养。对从皮肤刮屑中提取的DNA进行靶向11种马拉色菌属菌种的多重聚合酶链反应。
共研究了69例疑似PV病例。大多数患者来自大城市，在炎热潮湿的气候中工作。皮损的平均持续时间为18个月，大多数有斑疹和斑片状皮损。发现KOH和培养的敏感性分别为82.6%和91.3%。培养中分离出球形马拉色菌（n = 60，87%）和限制马拉色菌（n = 3，4.3%）。多重聚合酶链反应检测到85.5%的球形马拉色菌、5.8%的限制马拉色菌以及8.7%的球形马拉色菌和限制马拉色菌混合感染。多重聚合酶链反应在所有样本中均检测到马拉色菌。
多重聚合酶链反应可直接从皮肤标本中鉴定马拉色菌属菌种,无需进行培养。多重聚合酶链反应准确、可靠，且周转时间短。
