Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran.
Department of Cellular and Molecular Biotechnology, Institute of Biotechnology, Urmia University, Urmia, Iran.
Sci Rep. 2024 Sep 2;14(1):20323. doi: 10.1038/s41598-024-71234-4.
Metal oxide nanoparticles(NPs) contain unique properties which have made them attractive agents in cancer treatment. The CuO nanoparticles were green synthesized using walnut shell powder in different calcination temperatures (400°, 500°, 700°, and 900 °C). The CuO nanoparticles are characterized by FTIR, XRD, BET, SEM and DLS analyses. SEM and DLS analyses showed that by increasing the required calcination temperature for synthesizing the NPs, their size was increased. DPPH analysis displayed no significant anti-oxidative properties of the CuO NPs. The MTT analysis showed that all synthesized CuO NPs exhibited cytotoxic effects on MCF-7, HCT-116, and HEK-293 cell lines. Among the CuO NPs, the CuO-900 NPs showed the least cytotoxic effect on the HEK-293 cell line (IC = 330.8 µg/ml). Hoechst staining and real-time analysis suggested that the CuO-900 NPs induced apoptosis by elevation of p53 and Bax genes expression levels. Also, the CuO-900 NPs increased the Nrf-2 gene expression level in MCF-7 cells, despite the HCT-116 cells. As can be concluded from the results, the CuO-900 NPs exerted promising cytotoxic effects on breast and colon cancer cells.
金属氧化物纳米粒子（NPs）具有独特的性质，这使得它们成为癌症治疗的有吸引力的药物。使用核桃壳粉末在不同的煅烧温度（400°C、500°C、700°C 和 900°C）下绿色合成了氧化铜 NPs。通过傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）、BET、扫描电子显微镜（SEM）和动态光散射（DLS）分析对 CuO NPs 进行了表征。SEM 和 DLS 分析表明，通过增加合成 NPs 所需的煅烧温度，可以增加它们的尺寸。DPPH 分析显示 CuO NPs 没有显著的抗氧化性质。MTT 分析表明，所有合成的 CuO NPs 对 MCF-7、HCT-116 和 HEK-293 细胞系均表现出细胞毒性作用。在 CuO NPs 中，CuO-900 NPs 对 HEK-293 细胞系的细胞毒性作用最小（IC=330.8 µg/ml）。Hoechst 染色和实时分析表明，CuO-900 NPs 通过提高 p53 和 Bax 基因表达水平诱导细胞凋亡。此外，CuO-900 NPs 增加了 MCF-7 细胞中 Nrf-2 基因的表达水平，但对 HCT-116 细胞没有影响。从结果可以得出结论，CuO-900 NPs 对乳腺癌和结肠癌细胞表现出有前途的细胞毒性作用。
