Sherman Trent, Schlegel Patrick, Santuray Rodell, Zhang Zhaoyan, Long Jennifer L
Department of Head and Neck Surgery, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, California.
Department of Head and Neck Surgery, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, California; Greater Los Angeles Veterans Affairs Healthcare System, Los Angeles, California.
J Voice. 2024 Sep 2. doi: 10.1016/j.jvoice.2024.08.014.
Sex differences in response to trauma and physiologic stressors have been identified in numerous organ systems but have not yet been defined in the larynx. The objective of this study was to develop an endoscopic vocal fold injury model in rabbits and to compare structural and functional outcomes between male and female subjects.
Basic science study.
Two male and two female rabbits underwent unilateral endoscopic cordectomy. Animals were intubated with a size 3-0 neonatal endotracheal tube, and laryngoscopy was performed with a 4 mm Hopkins rod telescope. While visualizing, a 2 mm cupped forceps grasped and resected the mid-membranous portion of the right true vocal fold. Larynges were then harvested after 8weeks. Excised larynx phonation with high-speed videography and kymography was used to assess vibrational quality. Tissue elastic (Young's) modulus was measured by indentation.
Injured larynges phonated with fundamental frequencies between 237-415 Hz. In both males and females, the scarred vocal fold exhibited an increased Young's modulus compared to the contralateral nonoperated vocal fold. There were no notable differences in glottal closure pattern or vocal fold oscillation symmetry between sexes.
We have demonstrated a model for vocal fold scarring in rabbits. Vibrational and structural outcomes were similar between the examined male and female larynges.
在众多器官系统中已发现创伤和生理应激源反应存在性别差异，但喉部尚未明确。本研究的目的是建立兔内镜下声带损伤模型，并比较雄性和雌性受试者的结构和功能结果。
基础科学研究。
两只雄性和两只雌性兔接受单侧内镜下声带切除术。动物用3-0号新生儿气管内导管插管，并用4毫米Hopkins杆式望远镜进行喉镜检查。在可视化过程中，用2毫米杯状钳抓住并切除右真声带的膜中部。8周后取出喉部。切除的喉部发声用高速摄像和记波法评估振动质量。通过压痕测量组织弹性（杨氏）模量。
受伤喉部发声的基频在237-415赫兹之间。在雄性和雌性中，与对侧未手术的声带相比，瘢痕化的声带杨氏模量均增加。两性之间声门闭合模式或声带振动对称性无明显差异。
我们已证明兔声带瘢痕形成模型。所检查的雄性和雌性喉部的振动和结构结果相似。
