Hotta N, Kakuta H, Fukasawa H, Kimura M, Koh N, Iida M, Terashima H, Morimura T, Sakamoto N
Diabetologia. 1985 Mar;28(3):176-80. doi: 10.1007/BF00273868.
Streptozotocin-diabetic rats were maintained on a 72% fructose diet for 4 weeks and some were treated with an aldose reductase inhibitor (either alrestatin: 0.9 g . kg-1 . day-1 or ONO-2235: 50 mg . kg-1 . day-1). Fructose feeding significantly influenced the development of impaired motor nerve conduction velocity in the diabetic rats and this effect was positively correlated with sorbitol accumulation in the sciatic nerve of diabetic rats maintained on a fructose-rich diet. Treatment with ONO-2235, a new aldose reductase inhibitor, prevented both slowing of motor nerve conduction velocity and elevation of nerve sorbitol concentration. On the other hand, erythrocyte sorbitol levels were significantly correlated to those of the sciatic nerve (r = 0.86, p less than 0.001) and the retina (r = 0.91, p less than 0.001) in these animals. Thus, our findings suggest that increased polyol pathway activity may be related to the pathogenesis of diabetic neuropathy and erythrocyte sorbitol concentrations may prove a useful indicator for the presence of diabetic complications.
将链脲佐菌素诱导的糖尿病大鼠置于含72%果糖的饮食中饲养4周，部分大鼠用醛糖还原酶抑制剂治疗（阿雷司他汀：0.9 g·kg⁻¹·天⁻¹或ONO - 2235：50 mg·kg⁻¹·天⁻¹）。喂食果糖显著影响糖尿病大鼠运动神经传导速度受损的发展，且这种影响与食用富含果糖饮食的糖尿病大鼠坐骨神经中山梨醇的积累呈正相关。用新型醛糖还原酶抑制剂ONO - 2235治疗可防止运动神经传导速度减慢和神经山梨醇浓度升高。另一方面，在这些动物中，红细胞山梨醇水平与坐骨神经（r = 0.86，p < 0.001）和视网膜（r = 0.91，p < 0.001）的水平显著相关。因此，我们的研究结果表明，多元醇途径活性增加可能与糖尿病神经病变的发病机制有关，红细胞山梨醇浓度可能是糖尿病并发症存在的有用指标。
