Kunnas Kari, Vihinen-Ranta Maija, Leclerc Simon
Department of Biological and Environmental Science and Nanoscience Center, University of Jyväskylä, Jyvaskyla, Central Finland, Finland.
MicroPubl Biol. 2024 Aug 19;2024. doi: 10.17912/micropub.biology.001269. eCollection 2024.
During infection, Herpes simplex virus type 1 (HSV-1) alters the mitochondrial structure and function of the host cell. Live-cell imaging with fluorescent reporters revealed increased mitochondrial calcium and a transient ROS enrichment after HSV-1 infection. Notably, cells co-transfected with a calcium reporter displayed smaller viral replication compartments, while those with a ROS reporter exhibited average growth of viral replication compartments. Our findings suggest that the virus-induced increase in mitochondrial calcium, followed by an increased amount of bound calcium reporter, interferes with the progression of the infection.
在感染过程中，单纯疱疹病毒1型（HSV-1）会改变宿主细胞的线粒体结构和功能。使用荧光报告基因进行的活细胞成像显示，HSV-1感染后线粒体钙增加，活性氧短暂富集。值得注意的是，与钙报告基因共转染的细胞显示出较小的病毒复制区室，而与活性氧报告基因共转染的细胞则显示病毒复制区室平均生长。我们的研究结果表明，病毒诱导的线粒体钙增加，随后结合的钙报告基因数量增加，会干扰感染进程。
