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利用胶体金颗粒免疫电子显微镜技术对枯草芽孢杆菌休眠孢子中吡啶二羧酸进行超微结构定位

Ultrastructural localization of dipicolinic acid in dormant spores of Bacillus subtilis by immunoelectron microscopy with colloidal gold particles.

作者信息

Kozuka S, Yasuda Y, Tochikubo K

出版信息

J Bacteriol. 1985 Jun;162(3):1250-4. doi: 10.1128/jb.162.3.1250-1254.1985.

DOI:10.1128/jb.162.3.1250-1254.1985
PMID:3922946
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215911/
Abstract

The localization of dipicolinic acid in dormant spores of Bacillus subtilis was examined by an immunoelectron microscopy method with colloidal gold-immunoglobulin G complex. The colloidal gold particles were distributed mainly in the core regions of dormant spores and were not observed in those of germinated or autoclaved spores. This result clearly demonstrates that dipicolinic acid is localized in the cores of dormant spores.

摘要

采用胶体金-免疫球蛋白G复合物免疫电子显微镜方法，对枯草芽孢杆菌休眠孢子中吡啶二羧酸的定位进行了研究。胶体金颗粒主要分布在休眠孢子的核心区域，而在萌发或经高压灭菌的孢子中未观察到。这一结果清楚地表明，吡啶二羧酸定位于休眠孢子的核心。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65c/215911/17e6697d14d0/jbacter00223-0404-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65c/215911/9881aa7d02e3/jbacter00223-0403-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65c/215911/f94d202b4c24/jbacter00223-0403-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65c/215911/17e6697d14d0/jbacter00223-0404-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65c/215911/9881aa7d02e3/jbacter00223-0403-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65c/215911/f94d202b4c24/jbacter00223-0403-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65c/215911/17e6697d14d0/jbacter00223-0404-a.jpg

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