Tinglu G, Ghosh A, Ghosh B K
J Bacteriol. 1984 Aug;159(2):668-77. doi: 10.1128/jb.159.2.668-677.1984.
Subcellular distribution of the alkaline phosphatase of Bacillus licheniformis 749/C was determined by an immunoelectron microscopy method. Anti-alkaline phosphatase antibody labeled with 15- to 18-nm colloidal gold particles (gold-immunoglobulin G [IgG] complex) were used for the study. Both the plasma membrane and cytoplasmic material were labeled with the gold-IgG particles. These particles formed clusters in association with the plasma membrane; in contrast, in the cytoplasm the particles were largely dispersed, and only a few clusters were found. The gold-IgG binding was quantitatively estimated by stereological analysis of labeled, frozen thin sections. This estimation of a variety of control samples showed that the labeling was specific for the alkaline phosphatase. Cluster formation of the gold-IgG particles in association with the plasma membrane suggests that existence of specific alkaline phosphatase binding sites (receptors) in the plasma membrane of B. licheniformis 749/C.
通过免疫电子显微镜法测定了地衣芽孢杆菌749/C碱性磷酸酶的亚细胞分布。使用标记有15至18纳米胶体金颗粒（金-免疫球蛋白G [IgG]复合物）的抗碱性磷酸酶抗体进行该研究。质膜和细胞质物质均被金-IgG颗粒标记。这些颗粒与质膜结合形成簇；相反，在细胞质中颗粒大多分散，仅发现少数簇。通过对标记的冷冻薄切片进行体视学分析，定量估计金-IgG结合。对各种对照样品的这种估计表明，标记对碱性磷酸酶具有特异性。金-IgG颗粒与质膜结合形成簇表明地衣芽孢杆菌749/C质膜中存在特异性碱性磷酸酶结合位点（受体）。
