National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing, 100097, P. R. China.
State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing, 100097, P. R. China.
Sci Data. 2024 Sep 4;11(1):961. doi: 10.1038/s41597-024-03830-y.
Lettuce (Lactuca sativa L., Asteraceae) is one of the most important vegetable crops, known for its various horticultural types and significant morphological variation. The first reference genome of lettuce, a crisphead type (L. sativa var. capitata cv. Salinas), was previously released. Here, we reported a near-complete chromosome-level reference genome for looseleaf lettuce (L. sativa var. crispa). PacBio high-fidelity sequencing, Oxford Nanopore, and Hi-C technologies were employed to produce genome assembly. The final assembly is 2.59 Gb in length with a contig N50 of 205.47 Mb, anchored onto nine chromosomes, containing 14 recognizable telomeres and only 11 gaps. Repetitive sequences account for 77.11% of the genome, and 41,375 protein-coding genes were predicted, with 99.10% of these assigned functional annotations. This chromosome-level genome enriched genomic resources for various horticultural types of lettuce and will facilitate the characterization of morphological variation and genetic improvement in lettuce.
生菜（Lactuca sativa L.，菊科）是最重要的蔬菜作物之一，以其各种园艺类型和显著的形态变异而闻名。生菜的第一个参考基因组，脆叶型（L. sativa var. capitata cv. Salinas），之前已经发布。在这里，我们报道了一个散叶生菜（L. sativa var. crispa）的近完整染色体水平参考基因组。PacBio 高保真测序、Oxford Nanopore 和 Hi-C 技术被用于产生基因组组装。最终组装的长度为 2.59 Gb，contig N50 为 205.47 Mb，锚定在 9 条染色体上，包含 14 个可识别的端粒，只有 11 个缺口。重复序列占基因组的 77.11%，预测到 41375 个蛋白质编码基因，其中 99.10%被赋予了功能注释。这个染色体水平的基因组丰富了生菜各种园艺类型的基因组资源，将有助于生菜形态变异和遗传改良的研究。
