Truco Maria José, Ashrafi Hamid, Kozik Alexander, van Leeuwen Hans, Bowers John, Wo Sebastian Reyes Chin, Stoffel Kevin, Xu Huaqin, Hill Theresa, Van Deynze Allen, Michelmore Richard W
The Genome Center, University of California, Davis, California 95616.
Seed Biotechnology Center, University of California, Davis, California 95616.
G3 (Bethesda). 2013 Apr 9;3(4):617-631. doi: 10.1534/g3.112.004929.
We have generated an ultra-high-density genetic map for lettuce, an economically important member of the Compositae, consisting of 12,842 unigenes (13,943 markers) mapped in 3696 genetic bins distributed over nine chromosomal linkage groups. Genomic DNA was hybridized to a custom Affymetrix oligonucleotide array containing 6.4 million features representing 35,628 unigenes of Lactuca spp. Segregation of single-position polymorphisms was analyzed using 213 F recombinant inbred lines that had been generated by crossing cultivated Lactuca sativa cv. Salinas and L. serriola acc. US96UC23, the wild progenitor species of L. sativa The high level of replication of each allele in the recombinant inbred lines was exploited to identify single-position polymorphisms that were assigned to parental haplotypes. Marker information has been made available using GBrowse to facilitate access to the map. This map has been anchored to the previously published integrated map of lettuce providing candidate genes for multiple phenotypes. The high density of markers achieved in this ultradense map allowed syntenic studies between lettuce and Vitis vinifera as well as other plant species.
我们为菊科中一种具有重要经济价值的植物——生菜构建了一张超高密度遗传图谱，该图谱由12,842个单基因（13,943个标记）组成，分布在9个染色体连锁群的3696个遗传 bins 中。基因组DNA与定制的Affymetrix寡核苷酸阵列杂交，该阵列包含640万个特征，代表了莴苣属的35,628个单基因。使用通过栽培生菜品种萨利纳斯与野生祖先物种锯齿生菜US96UC23杂交产生的213个F重组自交系分析单核苷酸多态性的分离情况。利用重组自交系中每个等位基因的高复制水平来鉴定分配给亲本单倍型的单核苷酸多态性。已使用GBrowse提供标记信息，以方便访问该图谱。此图谱已锚定到先前发表的生菜综合图谱上，为多种表型提供了候选基因。这张超高密度图谱中实现的标记高密度使得能够对生菜与葡萄以及其他植物物种进行共线性研究。
