McHale Leah K, Truco Maria José, Kozik Alexander, Wroblewski Tadeusz, Ochoa Oswaldo E, Lahre Kirsten A, Knapp Steven J, Michelmore Richard W
The Genome Center and Department of Plant Sciences, University of California, Davis, CA 95616, USA.
Theor Appl Genet. 2009 Feb;118(3):565-80. doi: 10.1007/s00122-008-0921-1. Epub 2008 Nov 13.
Genbank and The Compositae Genome Project database, containing over 42,000 lettuce unigenes from Lactuca sativa cv. Salinas and L. serriola accession UC96US23 were mined to identify 702 candidate genes involved in pathogen recognition (RGCs), resistance signal transduction, defense responses, and disease susceptibility. In addition, to identify sequences representing additional sub-families of nucleotide binding site (NBS)-leucine-rich repeat encoding genes; the major classes of resistance genes (R-genes), NBS-encoding sequences were amplified by PCR using degenerate oligonucleotides designed to NBS sub-families specific to the subclass Asteridae, which includes the Compositae family. These products were cloned and sequenced resulting in 18 novel NBS sequences from cv. Salinas and 15 novel NBS sequences from UC96US23. Using a variety of marker technologies, 294 of the 735 candidate disease resistance genes were mapped in our primary mapping population, which consisted of 119 F7 recombinant inbred lines derived from an interspecific cross between cv. Salinas and UC96US23. Using markers shared across multiple genetic maps, 36 resistance phenotypic loci, including two new loci for resistance to downy mildew and two quantitative trait loci for resistance to anthracnose were positioned onto the reference map to provide a global view of the genomic architecture of disease resistance in lettuce and to identify candidate genes for resistance phenotypes. The majority but not all of the resistance phenotypes were genetically associated with RGCs.
对Genbank和菊科基因组计划数据库进行挖掘，该数据库包含来自生菜品种萨利纳斯（Lactuca sativa cv. Salinas）和刺叶莴苣种质UC96US23的42000多个生菜单基因，以鉴定702个参与病原体识别（RGCs）、抗性信号转导、防御反应和感病性的候选基因。此外，为了鉴定代表核苷酸结合位点（NBS）-富含亮氨酸重复序列编码基因其他亚家族的序列；抗性基因（R基因）的主要类别，使用针对菊亚纲（包括菊科）特有的NBS亚家族设计的简并寡核苷酸通过PCR扩增NBS编码序列。这些产物被克隆并测序，从萨利纳斯品种中获得了18个新的NBS序列，从UC96US23中获得了15个新的NBS序列。使用多种标记技术，在我们的初级作图群体中对735个候选抗病基因中的294个进行了定位，该群体由119个F7重组自交系组成，这些重组自交系来自萨利纳斯品种和UC96US23之间的种间杂交。使用多个遗传图谱共享的标记，将36个抗性表型位点，包括两个新的霜霉病抗性位点和两个炭疽病抗性数量性状位点定位到参考图谱上，以提供生菜抗病基因组结构的全局视图，并鉴定抗性表型的候选基因。大多数但不是所有的抗性表型在遗传上与RGCs相关。
