School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
Int J Biol Macromol. 2024 Nov;279(Pt 4):135267. doi: 10.1016/j.ijbiomac.2024.135267. Epub 2024 Sep 2.
This experiment examined the antiviral activity of polysaccharides from Sargassum fusiforme against respiratory syncytial virus (RSV) in vitro, including their mechanism of action and preliminary structural analysis. Four polysaccharides (SFP1, SFP2, SFP3, and SFP4) were purified from Sargassum fusiforme using a DEAE-52 cellulose column and an NW Super 150 gel column. CCK-8 and western blot were utilized to study the antiviral activities and mechanisms of the polysaccharides. Preliminary structural analysis was conducted using HPLC and NMR techniques. The findings suggest that SFP4 (120 kD) is an acidic chemical compound composed of 88.8 % total sugars, 0.13 % proteins, 10.8 % glucuronidic acids, and 21.1 % sulfates. It contains at least ten monosaccharides, primarily mannuronic acid and fucose. Among the four polysaccharides, SFP4 had the highest anti-RSV activity, with a therapeutic index (TI) exceeding 139. SFP4 exhibited noteworthy antiviral efficacy in both upper and lower respiratory cells that were infected, especially when administered as a prophylactic treatment 2 h in advance. Furthermore, SFP4 showed a dose-dependent antiviral effect, with the highest therapeutic index (TI > 320) observed at a concentration of 7.81 μg·mL during the prophylactic phase. It was speculated that SFP4's antiviral effect is due to its ability to inhibit the attachment of G-proteins to cells.
本实验研究了马尾藻多糖对呼吸道合胞病毒（RSV）的体外抗病毒活性，包括其作用机制和初步结构分析。采用 DEAE-52 纤维素柱和 NW Super 150 凝胶柱从马尾藻中分离得到 4 种多糖（SFP1、SFP2、SFP3 和 SFP4）。采用 CCK-8 法和 Western blot 法研究了多糖的抗病毒活性及其作用机制。采用 HPLC 和 NMR 技术对其进行了初步的结构分析。结果表明，SFP4（120kD）是一种酸性化合物，由 88.8%的总糖、0.13%的蛋白质、10.8%的葡萄糖醛酸和 21.1%的硫酸组成。它至少含有十种单糖，主要为甘露糖醛酸和岩藻糖。在这四种多糖中，SFP4 对 RSV 的抑制活性最高，治疗指数（TI）超过 139。SFP4 对感染的上呼吸道和下呼吸道细胞均具有显著的抗病毒作用，尤其是作为预防性治疗提前 2 小时给药时效果更为明显。此外，SFP4 表现出剂量依赖性的抗病毒作用，在预防性阶段浓度为 7.81μg·mL-1 时具有最高的治疗指数（TI>320）。推测 SFP4 的抗病毒作用可能与其抑制 G 蛋白与细胞结合的能力有关。
