Ma Rongxing, Gao Xiaokang, Jin Yangyang, Wang Xiaolong, Li Ruifeng, Qiao Ruiqi, Wang Xinliang, Liu Dayong, Xie Zhitao, Wang Limin, Zhang Jingyu, Xu Weiguo, Hu Yongcheng
Clinical School/College of Orthopedics, Tianjin Medical University, Tianjin, China.
Tianjin Hospital, Tianjin University, Tianjin, China.
Front Med (Lausanne). 2024 Aug 21;11:1417930. doi: 10.3389/fmed.2024.1417930. eCollection 2024.
Decellularized allograft tendons are highly regarded for their accessibility and the reduced risk of immune rejection, making them a promising choice for grafting due to their favorable characteristics. However, effectively integrating reconstructed tendons with host bone remains a significant clinical challenge.
This study aims to investigate the relationship between the duration of tendon exposure to trypsin and its impact on tendon biomechanical properties and healing capacity.
Morphological assessments and biochemical quantifications were conducted. Allograft tendons underwent heterotopic transplantation into the anterior cruciate ligament (ACL) in a rabbit model, with specimens harvested 6 weeks post-surgery for a comparative analysis of cell adhesion strength and mechanical performance. Duration-response curves were constructed using maximum stress and cell adhesion quantity as primary indicators.
The trypsin treatment enhanced cell adhesion on the tendon surface. Adhesion rates in the control group vs. the experimental groups were as follows: 3.10 ± 0.56% vs. 4.59 ± 1.51%, 5.36 ± 1.24%, 6.12 ± 1.98%, and 8.27 ± 2.34% ( = 6.755, = 0.001). However, increasing treatment duration led to a decline in mechanical properties, with the ultimate load (N) in the control vs. experimental groups reported as 103.30 ± 10.51 vs. 99.59 ± 4.37, 93.15 ± 12.38, 90.42 ± 7.87, and 82.68 ± 6.89, = 4.125 ( = 0.013).
The findings reveal an increasing trend in adhesion effectiveness with prolonged exposure duration, while mechanical strength declines. The selection of the optimal processing duration should involve careful consideration of the benefits derived from both outcomes.
脱细胞同种异体肌腱因其易获取性和免疫排斥风险降低而备受关注，因其良好特性使其成为移植的有前景选择。然而，有效将重建肌腱与宿主骨整合仍是一项重大临床挑战。
本研究旨在探讨肌腱暴露于胰蛋白酶的持续时间与其对肌腱生物力学性能和愈合能力影响之间的关系。
进行了形态学评估和生化定量分析。同种异体肌腱在兔模型中异位移植到前交叉韧带（ACL），术后6周采集标本，用于细胞黏附强度和力学性能的比较分析。以最大应力和细胞黏附量作为主要指标构建持续时间 - 反应曲线。
胰蛋白酶处理增强了肌腱表面的细胞黏附。对照组与实验组的黏附率如下：3.10±0.56% 对 4.59±1.51%、5.36±1.24%、6.12±1.98% 和 8.27±2.34%（F = 6.755，P = 0.001）。然而，处理持续时间增加导致力学性能下降，对照组与实验组的极限载荷（N）分别为 103.30±10.51 对 99.59±4.37、93.15±12.38、90.42±7.87 和 82.68±6.89，F = 4.125（P = 0.013）。
研究结果表明，随着暴露持续时间延长，黏附有效性呈增加趋势，而机械强度下降。最佳处理持续时间的选择应仔细权衡这两种结果带来的益处。
