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Point mutations in the 5' ICR and anticodon region of a Drosophila tRNAArg gene decrease in vitro transcription.

作者信息

Stewart T S, Söll D, Sharp S

出版信息

Nucleic Acids Res. 1985 Jan 25;13(2):435-47. doi: 10.1093/nar/13.2.435.

DOI:10.1093/nar/13.2.435
PMID:3923433
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC341006/
Abstract

We have examined the effects of various nucleotide substitutions in a Drosophila tRNAArg gene on in vitro transcription and stable transcription complex formation in Drosophila KcO and HeLa cell extracts. Substitutions in positions encoding the invariant G18 and G19 residues resulted in decreased transcription, however, the moderate decreases indicate that these nucleotides are not obligatory promoter recognition sites. An A21 to C21 mutation had no effect on transcription levels using homologous extract however, this mutant displayed decreased transcriptional abilities in HeLa cell extract. Nucleotide substitutions within the sequence encoding the anticodon led to a decrease in the transcription activity but not in the ability to form a stable transcription complex.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc3a/341006/03f476e5a7c8/nar00296-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc3a/341006/9c77aebb9d1b/nar00296-0127-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc3a/341006/2a31f6b817e0/nar00296-0129-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc3a/341006/666256a534ca/nar00296-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc3a/341006/03f476e5a7c8/nar00296-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc3a/341006/9c77aebb9d1b/nar00296-0127-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc3a/341006/2a31f6b817e0/nar00296-0129-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc3a/341006/666256a534ca/nar00296-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc3a/341006/03f476e5a7c8/nar00296-0131-a.jpg

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本文引用的文献

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Two conserved sequence blocks within eukaryotic tRNA genes are major promoter elements.真核生物tRNA基因中的两个保守序列块是主要的启动子元件。
Nature. 1981 Dec 17;294(5842):626-31. doi: 10.1038/294626a0.
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A split promoter for a eucaryotic tRNA gene.一种真核生物tRNA基因的分裂启动子。
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Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates.体内tRNA基因的转录：单链模板与双链模板的比较
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Transcription of eucaryotic tRNA1met and 5SRNA genes by RNA polymerase III is blocked by base mismatches in the intragenic control regions.RNA聚合酶III对真核生物tRNA1met和5SRNA基因的转录会被基因内控制区域中的碱基错配所阻断。
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A yeast tRNA(Arg) gene can act as promoter for a 5' flank deficient, non-transcribable tRNA(SUP)6 gene to produce biologically active suppressor tRNA.一个酵母tRNA（精氨酸）基因可作为5'侧翼缺失、不可转录的tRNA（SUP）6基因的启动子，以产生具有生物活性的抑制性tRNA。
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Yeast RNase P: catalytic activity and substrate binding are separate functions.酵母核糖核酸酶P：催化活性与底物结合是各自独立的功能。
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Competitive and cooperative functioning of the anterior and posterior promoter elements of an Alu family repeat.Alu家族重复序列前后启动子元件的竞争与协同作用。
Mol Cell Biol. 1986 Jun;6(6):2041-52. doi: 10.1128/mcb.6.6.2041-2052.1986.
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Sequences between the internal control regions of tRNAArg of Drosophila melanogaster influence stimulation of transcription of the 5' flanking DNA.黑腹果蝇tRNAArg内部控制区域之间的序列影响5'侧翼DNA转录的刺激。
Nucleic Acids Res. 1988 Mar 25;16(6):2585-99. doi: 10.1093/nar/16.6.2585.
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Construction of an opal suppressor by oligonucleotide-directed mutagenesis of a Saccharomyces cerevisiae tRNA(Trp) gene.通过对酿酒酵母tRNA（Trp）基因进行寡核苷酸定向诱变构建乳白抑制基因。
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Internal control regions for transcription of eukaryotic tRNA genes.真核生物tRNA基因转录的内部控制区域。
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