Nurmakhanov T, Tukhanova N, Sayakova Z, Sadovskaya V, Shevtsov A, Tokmurziyeva G, Turebekov N
Laboratory of Natural-Focal Viral Infections, M.Aikymbayev's National Scientific Center for Especially Dangerous Infections, Almaty, Kazakhstan.
Department of Epizootology of Especially Dangerous Infections with the Museum and Insectarium, M.Aikymbayev's National Scientific Center for Especially Dangerous Infections, Almaty, Kazakhstan.
Front Epidemiol. 2024 Aug 22;4:1310071. doi: 10.3389/fepid.2024.1310071. eCollection 2024.
The natural foci of Crimean-Congo haemorrhagic fever (CCHF) in Kazakhstan are geographically located in the southern regions of the country (Kyzylorda, Turkestan and Zhambyl regions), where the infection of ticks with the CCHF virus predominantly reside, tick species composition and the number of vectors are monitored annually. The objective of our research was to investigate the genetic variants of the CCHF virus in the southern endemic regions, as well as to monitor the spread of the CCHF virus in the western regions of the country (Aktobe, Atyrau and Mangystau regions). In total, 974 (216 pools) ticks from the western regions and 3527 (583 pools) ticks from the southern regions collected during 2021-2022 were investigated. The presence of CCHF virus was detected by real-time reverse transcription PCR (qRT- PCR) in 1 pool out of 799 pools (0.12%) with ticks captured in the CCHF-endemic Kyzylorda region. In the western regions, CCHF virus was not detected in ticks. The sequencing of incomplete fragments of the S, M and L segments of the CCHF virus in the detected virus was identified as genotype . Phylogenetic analysis showed that the isolate obtained in this study is grouped with the isolate from a patient with CCHF, which we reported in 2015 (KX129738 Genbank). Our findings highlight the importance of including sequencing in the annual monitoring system for better understanding the evolution of the CCHF virus in the study areas of our country.
哈萨克斯坦克里米亚-刚果出血热（CCHF）的自然疫源地在地理位置上位于该国南部地区（克孜勒奥尔达、突厥斯坦和江布尔地区），CCHF病毒主要感染的蜱虫就栖息于此，每年都会监测蜱虫的种类组成和媒介数量。我们研究的目的是调查该国南部流行地区CCHF病毒的基因变异情况，并监测CCHF病毒在该国西部地区（阿克托别、阿特劳和曼格斯套地区）的传播情况。2021年至2022年期间，共对西部地区的974只（216组）蜱虫和南部地区的3527只（583组）蜱虫进行了调查。通过实时逆转录PCR（qRT-PCR）检测发现，在克孜勒奥尔达地区（CCHF流行区）捕获的799组蜱虫中有1组（0.12%）检测到CCHF病毒。在西部地区的蜱虫中未检测到CCHF病毒。对检测到的病毒中CCHF病毒S、M和L片段的不完整片段进行测序，确定为基因型。系统发育分析表明，本研究获得的分离株与我们在2015年报告的一名CCHF患者的分离株（Genbank登录号：KX129738）归为一组。我们的研究结果凸显了在年度监测系统中纳入测序的重要性，以便更好地了解我国研究区域内CCHF病毒的进化情况。
