Corvazier E, Maclouf J
Biochim Biophys Acta. 1985 Jul 9;835(2):315-21. doi: 10.1016/0005-2760(85)90287-5.
The effect of ten flavonoids was studied on the stimulation of washed human platelets by either arachidonic acid or thrombin. The oxygenated metabolites released were analyzed by radioimmunoassay, glass-capillary-column gas chromatography and high-pressure liquid chromatography. No effect was evidenced for naringenin, rutinose and phloridzin up to 1000 microM. Thromboxane B2 and 12-hydroxyeicosatetraenoic acid production was depressed simultaneously by all other compounds at different IC50. When tested for their effect on reversibility, however, cyclooxygenase and lipoxygenase inhibition was found to be different depending upon the flavonoid used. All compounds, except morin and rutin, inhibited platelet aggregation and [14C]serotonin release with parallel inhibition of thromboxane synthesis when tested on arachidonic acid-induced platelet-rich plasma stimulation. Some flavonoids inhibited the metabolism of human neutrophils stimulated by ionophore A23187 as assessed by high-performance liquid chromatography. Our results show that flavonoids interfere with the different oxidative metabolisms of arachidonic acid. No clearcut specificity could be found between one compound and one metabolic pathway.
研究了十种黄酮类化合物对花生四烯酸或凝血酶刺激洗涤过的人血小板的影响。通过放射免疫测定、玻璃毛细管柱气相色谱法和高压液相色谱法分析释放的氧化代谢产物。高达1000微摩尔时，柚皮素、芸香糖和根皮苷未显示出任何作用。所有其他化合物在不同的半数抑制浓度（IC50）下同时抑制血栓素B2和12-羟基二十碳四烯酸的产生。然而，在测试它们对可逆性的影响时，发现环氧化酶和脂氧合酶的抑制作用因所用黄酮类化合物而异。在花生四烯酸诱导的富含血小板血浆刺激试验中，除桑色素和芦丁外，所有化合物均抑制血小板聚集和[14C]血清素释放，并同时抑制血栓素合成。通过高效液相色谱法评估，一些黄酮类化合物抑制离子载体A23187刺激的人中性粒细胞的代谢。我们的结果表明，黄酮类化合物干扰花生四烯酸的不同氧化代谢。在一种化合物和一种代谢途径之间未发现明确的特异性。
