Swinnen Siene, de Azambuja Francisco, Parac-Vogt Tatjana N
Department of Chemistry, KU Leuven, Celestijnenlaan 200F, Leuven, 3001, Belgium.
Adv Healthc Mater. 2025 Mar;14(8):e2401547. doi: 10.1002/adhm.202401547. Epub 2024 Sep 9.
Metal-organic frameworks (MOFs) have the potential to revolutionize the biotechnological and medical landscapes due to their easily tunable crystalline porous structure. Herein, the study presents MOFs' potential impact on proteomics, unveiling the diverse roles MOFs can play to boost it. Although MOFs are excellent catalysts in other scientific disciplines, their role as catalysts in proteomics applications remains largely underexplored, despite protein cleavage being of crucial importance in proteomics protocols. Additionally, the study discusses evolving MOF materials that are tailored for proteomics, showcasing their structural diversity and functional advantages compared to other types of materials used for similar applications. MOFs can be developed to seamlessly integrate into proteomics workflows due to their tunable features, contributing to protein separation, peptide enrichment, and ionization for mass spectrometry. This review is meant as a guide to help bridge the gap between material scientists, engineers, and MOF chemists and on the other side researchers in biology or bioinformatics working in proteomics.
金属有机框架（MOFs）因其易于调节的晶体多孔结构，有潜力变革生物技术和医学领域。在此，本研究展示了MOFs对蛋白质组学的潜在影响，揭示了MOFs在推动蛋白质组学发展中可发挥的多种作用。尽管MOFs在其他科学领域是出色的催化剂，但在蛋白质组学应用中，其作为催化剂的作用在很大程度上仍未得到充分探索，尽管蛋白质裂解在蛋白质组学实验方案中至关重要。此外，该研究讨论了为蛋白质组学量身定制的不断发展的MOF材料，展示了它们与用于类似应用的其他类型材料相比的结构多样性和功能优势。由于其可调节的特性，MOFs可以被开发成无缝集成到蛋白质组学工作流程中，有助于蛋白质分离、肽富集以及用于质谱分析的离子化。这篇综述旨在作为一个指南，帮助弥合材料科学家、工程师、MOF化学家与从事蛋白质组学研究的生物学或生物信息学领域的研究人员之间的差距。
