Melamed M D, Gordon J, Ley S J, Edgar D, Hughes-Jones N C
Eur J Immunol. 1985 Jul;15(7):742-6. doi: 10.1002/eji.1830150720.
The exploitation of Epstein-Barr virus transformation to generate human lymphoblastoid clones (LCL) producing antibody of predefined specificity has proved highly inefficient. Observations reported here on a cloned LCL producing anti-Rhesus(D) may provide an explanation for the low success rate. Over a few months this clone manifested a progressive loss of capacity to maintain growth in culture. Evidence consistent with terminal differentiation to a cell with the properties of a nonproliferating plasma cell was obtained. These late cells differed from those in the earlier actively cycling phase in that they were no longer able to respond to autostimulatory growth factors although they continued to produce them. This irreversible senescence leading to the death of the clone may be a common feature of virally transformed B cells and this would explain many of the difficulties encountered on this route to the production of human monoclonal antibodies.
利用爱泼斯坦-巴尔病毒转化来生成产生预定特异性抗体的人淋巴母细胞样克隆（LCL）已被证明效率极低。此处报道的关于一个产生抗恒河猴（D）抗体的克隆LCL的观察结果可能为低成功率提供一个解释。在几个月的时间里，这个克隆表现出在培养中维持生长能力的逐渐丧失。获得了与终末分化为具有非增殖性浆细胞特性的细胞相一致的证据。这些后期细胞与早期活跃循环阶段的细胞不同，因为它们虽然继续产生自刺激生长因子，但不再能够对其作出反应。这种导致克隆死亡的不可逆衰老可能是病毒转化的B细胞的一个共同特征，这将解释在这条生产人单克隆抗体的途径中遇到的许多困难。
