Gorny M K, Gianakakos V, Sharpe S, Zolla-Pazner S
Department of Pathology, New York University Medical Center, New York 10016.
Proc Natl Acad Sci U S A. 1989 Mar;86(5):1624-8. doi: 10.1073/pnas.86.5.1624.
Based on the finding that cells producing antibodies to human immunodeficiency virus (HIV) circulate in the peripheral blood of HIV-infected individuals, attempts were made to immortalize such B cells with Epstein-Barr virus. Mononuclear cells from 58 HIV-seropositive subjects at various stages of HIV infection were transformed, and anti-HIV cell lines were derived from 4 subjects, all of whom were in early stages of infection. Seven of these cell lines have been stable with respect to antibody production for up to 15 months. Three lines are producing IgG antibody to the 41-kDa HIV transmembrane glycoprotein gp41 and 4 produce IgG antibodies to the 24-kDa HIV core protein p24, its precursors and a breakdown product. The antibodies are reactive by ELISA, by radioimmunoprecipitation, and by Western blot, demonstrating the feasibility of producing multiple stable cell lines synthesizing human monoclonal antibodies to HIV by immortalization of peripheral blood cells with Epstein-Barr virus.
基于在感染人类免疫缺陷病毒（HIV）个体的外周血中存在产生抗HIV抗体细胞的这一发现，人们尝试用爱泼斯坦-巴尔病毒使此类B细胞永生化。对处于HIV感染不同阶段的58名HIV血清阳性受试者的单核细胞进行了转化，从4名受试者（均处于感染早期）中获得了抗HIV细胞系。其中7个细胞系在抗体产生方面已稳定长达15个月。3个细胞系产生针对41-kDa HIV跨膜糖蛋白gp41的IgG抗体，4个细胞系产生针对24-kDa HIV核心蛋白p24及其前体和一种降解产物的IgG抗体。这些抗体通过ELISA、放射免疫沉淀和蛋白质印迹法均呈阳性反应，这表明通过用爱泼斯坦-巴尔病毒使外周血细胞永生化来产生多种合成抗HIV人单克隆抗体的稳定细胞系是可行的。
