Sakthivel Srudhika, Girija A S Smiline, Priyadharsini Vijayashree J, Parameshwari Kannika K
Department of Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS) Deemed University, Chennai, IND.
Cureus. 2024 Aug 6;16(8):e66335. doi: 10.7759/cureus.66335. eCollection 2024 Aug.
Objectives Mutacins are potent virulent factors attributing to the virulence in leading to oro-dental diseases, and oral potentially malignant disorders (OPMDs) are considered a premalignant condition of the oro-mucosal layers in the oral cavity. The purpose of this study was to phenotypically characterize from the clinical samples of patients with OPMD and to assess the frequency of mutacin genes in comparison with healthy individuals. Methods Saliva samples (n=60) were collected from three different groups and the samples were incubated at 37°C for 48 hours in Mutans-Sanguis agar. After incubation, the isolates were identified phenotypically for and the frequency of mutacin genes and its types were assessed by polymerase chain reaction (PCR). Results was found to be more prevalent in the OPMD cases (45%) followed by healthy individuals with caries (15%). Mutacin genes were expressed in all the groups except Group 3 (healthy individuals) without caries. Mutacin I was expressed the highest in Group 1 and Group 2 with 88% and 62.5, respectively, and mutacin III was expressed the least in all groups with 0% expression. Conclusion The findings of the study show the presence of mutacin gene types in the clinical strains of in association with OPMD and caries. Further experimental evidence may be required to assess the frequency and to design a novel drug targeting the same.
目的 变链菌素是导致口腔牙病的强效致病因素，口腔潜在恶性疾病（OPMD）被认为是口腔黏膜层的一种癌前状态。本研究的目的是对OPMD患者的临床样本进行表型特征分析，并与健康个体比较评估变链菌素基因的频率。方法 从三个不同组收集唾液样本（n = 60），并将样本在变形链球菌-血琼脂中于37°C孵育48小时。孵育后，对分离株进行表型鉴定，并通过聚合酶链反应（PCR）评估变链菌素基因的频率及其类型。结果 发现变链菌素在OPMD病例中更普遍（45%），其次是患有龋齿的健康个体（15%）。除第3组（无龋齿的健康个体）外，所有组均表达变链菌素基因。变链菌素I在第1组和第2组中表达最高，分别为88%和62.5%，变链菌素III在所有组中表达最少，表达率为0%。结论 研究结果表明，与OPMD和龋齿相关的变形链球菌临床菌株中存在变链菌素基因类型。可能需要进一步的实验证据来评估其频率并设计针对其的新型药物。
