Srivastava Kumar Chandan, Austin Ravi David, Shrivastava Deepti
Department of Oral Basic and Clinical Sciences, Division of Oral Diagnosis, Faculty of Dentistry, Qassim Private Colleges, Qassim Province, Kingdom of Saudi Arabia.
Division of Oral Medicine and Radiology, Rajah Muthiah Dental College and Hospital, Annamalai University, Tamil Nadu, India.
Dent Res J (Isfahan). 2016 Mar-Apr;13(2):181-7. doi: 10.4103/1735-3327.178210.
Imbalances between the oxidant-antioxidant status have been implicated in the pathogenesis of several diseases, including cancer. The aim of this study was to evaluate the extent of lipid peroxidation and antioxidants in the tissue samples of oral squamous cell carcinoma (OSCC) patients of different clinical stages in comparison with the healthy controls.
A case-control study was designed with 20 new histopathologically proven oral carcinoma patients and an equal number of age, sex, and tobacco chewing habit matched healthy subjects. Their tissue samples were subjected to evaluation of lipid peroxidation product and antioxidant enzymes, namely, superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and glutathione peroxidase (GPx) using spectrophotometric methods. The data are expressed as mean ± standard deviation. The statistical comparisons between the study groups were performed by independent Student's unpaired t-test and one-way analysis of variance. Post-hoc analysis was performed for within study group comparisons. Karl Pearson correlation was performed for the biochemical parameters within the group and between the groups. For statistically significant correlations, simple linear regression was performed using SPSS (α=0.05).
Significant reduction in lipid peroxidation (P < 0.001) SOD and CAT (P < 0.001) was observed in the tissue of OSCC patients as compared with the healthy controls. On the other hand, reduced GSH and GPx were significantly increased in tumor samples.
Reduced lipid peroxidation and increased activity of reduced GSH and GPx provides the suitable environment for the local growth and invasion of the tumor and metastasis in the later stages. Among the antioxidant enzymes, GSH reductase appears to have a profound role in carcinogenesis and thus it can be considered as potential prognostic marker.
氧化-抗氧化状态失衡与包括癌症在内的多种疾病的发病机制有关。本研究的目的是评估不同临床分期的口腔鳞状细胞癌（OSCC）患者组织样本中的脂质过氧化程度和抗氧化剂水平，并与健康对照进行比较。
设计了一项病例对照研究，纳入20例新确诊的经组织病理学证实的口腔癌患者以及数量相等、年龄、性别和咀嚼烟草习惯相匹配的健康受试者。使用分光光度法对他们的组织样本进行脂质过氧化产物和抗氧化酶的评估，这些抗氧化酶包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、还原型谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GPx）。数据以平均值±标准差表示。研究组之间的统计比较采用独立样本t检验和单因素方差分析。对研究组内进行事后分析。对组内和组间的生化参数进行Karl Pearson相关性分析。对于具有统计学意义的相关性，使用SPSS进行简单线性回归（α=0.05）。
与健康对照相比，OSCC患者组织中的脂质过氧化（P<0.001）、SOD和CAT（P<0.001）显著降低。另一方面，肿瘤样本中还原型GSH和GPx显著升高。
脂质过氧化降低以及还原型GSH和GPx活性增加为肿瘤的局部生长、侵袭及后期转移提供了适宜的环境。在抗氧化酶中，GSH还原酶似乎在致癌过程中起重要作用，因此可被视为潜在的预后标志物。
