Srivastava Kumar Chandan, Shrivastava Deepti
Department of Oral Basic and Clinical Sciences, Qaseem Private Colleges, Faculty of Dentistry, Qassim Province, Kingdom of Saudi Arabia.
J Int Soc Prev Community Dent. 2016 Dec;6(Suppl 3):S213-S218. doi: 10.4103/2231-0762.197195.
Imbalances between the oxidant-antioxidant status have been implicated in the pathogenesis of several diseases, including oral cancer. Mostly, all oral cancer lesions are preceded by a stage of premalignancy. The present study aims to evaluate lipid peroxidation and antioxidant status in the venous blood of patients with different clinicopathologic stages of leukoplakia.
A case control study was designed with the inclusion of 20 new cases of histopathologically proven leukoplakia of various clinical stages along with an equal number of positive and negative control individuals. The concentrations of thiobarbituric acid reactive substances and the activities of the antioxidant enzymes, namely superoxide dismutase, reduced glutathione, glutathione peroxidase, and catalase, were estimated in plasma using spectrophotometric methods. The data are expressed as mean ± SD. The statistical comparisons between and within the study groups were performed by one-way analysis of variance followed by analysis. Karl Pearson correlation was performed for the biochemical parameters within the group and between the groups. For statistically significant correlations, simple linear regression was performed.
Significant enhanced lipid peroxidation ( < 0.001) with a decrease in antioxidants ( < 0.001) was observed in the venous blood of leukoplakia patients compared with positive as well as negative controls. Accordingly, significant ( < 0.001) pattern of progression in thiobarbituric acid reactive substances levels was observed at various clinical stages among patients of both control groups. Among enzymes, glutathione showed significant ( < 0.001) reduction along the stages on comparison with two control groups.
Enhanced lipid peroxidation and compromised antioxidant defense in plasma indicate the development of oxidative stress. Among the antioxidant enzymes, reduced glutathione and glutathione Pperoxidase may play a decisive role in malignant transformation.
氧化-抗氧化状态失衡与包括口腔癌在内的多种疾病的发病机制有关。大多数口腔癌病变之前都有一个癌前阶段。本研究旨在评估不同临床病理阶段白斑患者静脉血中的脂质过氧化和抗氧化状态。
设计了一项病例对照研究，纳入20例经组织病理学证实的不同临床阶段的白斑新病例，以及数量相等的阳性和阴性对照个体。采用分光光度法测定血浆中硫代巴比妥酸反应性物质的浓度以及抗氧化酶超氧化物歧化酶、还原型谷胱甘肽、谷胱甘肽过氧化物酶和过氧化氢酶的活性。数据以平均值±标准差表示。研究组之间和组内的统计比较采用单因素方差分析，随后进行分析。对组内和组间的生化参数进行Karl Pearson相关性分析。对于具有统计学意义的相关性，进行简单线性回归分析。
与阳性和阴性对照相比，白斑患者静脉血中脂质过氧化显著增强（<0.001），抗氧化剂减少（<0.001）。因此，在两个对照组患者的不同临床阶段，硫代巴比妥酸反应性物质水平均观察到显著（<0.001）的进展模式。在酶中，与两个对照组相比，谷胱甘肽在各阶段均显示出显著（<0.001）降低。
血浆中脂质过氧化增强和抗氧化防御受损表明氧化应激的发展。在抗氧化酶中，还原型谷胱甘肽和谷胱甘肽过氧化物酶可能在恶性转化中起决定性作用。
