黑腹果蝇组织特异性α-淀粉酶表达的调控基因和元件的遗传定位与作用。

Genetic Localization and Action of Regulatory Genes and Elements for Tissue-Specific Expression of alpha-Amylase in DROSOPHILA MELANOGASTER.

机构信息

Department of Population and Evolutionary Biology, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands.

出版信息

Genetics. 1986 Dec;114(4):1131-45. doi: 10.1093/genetics/114.4.1131.

DOI:10.1093/genetics/114.4.1131

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1203032/

Abstract

Regulation of tissue-specific alpha-amylase (Amy) expression in Drosophila melanogaster was investigated with a newly developed method that detects the distribution of alpha-amylase allozymes in midguts of single adults or third-instar larvae. Trans regulation was found for alpha-amylase production in the posterior midgut (PMG) of adults, whereas cis regulation was demonstrated for the larval midgut. Independent regulation of components of the duplicated Amy locus was found in larvae. Recombination between the components of the Amy locus did not result in separation of the putative, very closely linked (less than 0.1 cM) cis-acting regulatory elements for alpha-amylase expression in the anterior midgut (AMG) of larvae. The expression of one of the components of the duplicated Amy locus in the AMG of larvae was influenced by a regulatory gene that was mapped at 2-79.1. alpha-Amylase expression in the adult PMG was controlled by a trans-acting regulatory gene localized at 2-79.0, in agreement with the data of Abraham and Doane.

摘要

我们采用一种新的方法研究了黑腹果蝇组织特异性α-淀粉酶（Amy）表达的调控，该方法可检测单个成虫或三龄幼虫中α-淀粉酶同工酶的分布。结果发现，成虫后肠（PMG）中的α-淀粉酶产生受到反式调控，而幼虫中则存在顺式调控。在幼虫中，发现了重复 Amy 基因座的成分的独立调控。Amy 基因座的成分之间的重组并没有导致幼虫前肠（AMG）中α-淀粉酶表达的假定、非常紧密连锁（小于 0.1 cM）的顺式作用调控元件分离。重复 Amy 基因座的一个成分在幼虫 AMG 中的表达受到一个位于 2-79.1 的调控基因的影响。成虫 PMG 中的α-淀粉酶表达受定位于 2-79.0 的反式作用调控基因控制，这与 Abraham 和 Doane 的数据一致。

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本文引用的文献

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