Leung J, Addison W R, Delaney A D, MacKay R M, Miller R C, Spiegelman G B, Grigliatti T A, Tener G M
Gene. 1985;34(2-3):207-17. doi: 10.1016/0378-1119(85)90129-5.
Drosophila tRNAVal3b genes have been analyzed with respect to their nucleotide sequence and in vitro transcription efficiency. Plasmid pDt78R contains a single tRNA gene derived from the major tRNAVal3b gene cluster at chromosome band 84D. Its sequence corresponds to that of the tRNAVal3b. Two other plasmids, pDt41R and pDt48, each contain a tRNAVal3b-like gene from the minor tRNAVal3b gene cluster at chromosome bands 90BC. They contain the expected CAC anticodon, but their sequence differs from the tRNA at four positions. In homologous cell-free extracts, the tRNAVal3b variant genes in pDt41R and pDt48 are transcribed an order of magnitude more efficiently than the tRNAVal3b gene in pDt78R. However, the variant genes do not appear to contribute significantly to the in vivo tRNA pool [Larsen et al.: Mol. Gen. Genet. 185 (1982) 390-396]. We propose the term allogenes to describe families of related DNA sequences that may code for variant forms of a standard tRNA isoaccepting species.
对果蝇tRNAVal3b基因的核苷酸序列和体外转录效率进行了分析。质粒pDt78R含有一个来自染色体带84D处主要tRNAVal3b基因簇的单个tRNA基因。其序列与tRNAVal3b的序列相对应。另外两个质粒pDt41R和pDt48，每个都含有一个来自染色体带90BC处次要tRNAVal3b基因簇的类似tRNAVal3b的基因。它们含有预期的CAC反密码子，但它们的序列在四个位置上与tRNA不同。在同源无细胞提取物中，pDt41R和pDt48中的tRNAVal3b变体基因的转录效率比pDt78R中的tRNAVal3b基因高一个数量级。然而，这些变体基因似乎对体内tRNA库没有显著贡献[拉森等人：《分子遗传学与普通遗传学》185（1982）390 - 396]。我们提出“同种基因”这一术语来描述可能编码标准tRNA同功受体种类变体形式的相关DNA序列家族。
