Larsen T M, Miller R C, Spiegelman G B, Hayashi S, Tener G M, Sinclair D A, Grigliatti T A
Mol Gen Genet. 1982;185(3):390-6. doi: 10.1007/BF00334129.
Transfer RNA was extracted from 50-300 mg of adult flies and specifically labeled in vitro. The level of individual isoacceptors was quantitated by efficient annealing to Drosophila tRNA genes carried on recombinant DNA plasmids immobilized on nitrocellulose filters. The level of tRNAVal3b in the tRNA isolated from flies deficient in the major tRNAVal3b loci has been examined. The results show that deletion of the major tRNAVal3b loci resulted in a reduction of approximately 50% in the level of tRNAVal3b but did not produce the Minute phenotype; furthermore the effects of deficiencies at two loci were approximately additive.
从50 - 300毫克成年果蝇中提取转移RNA，并在体外进行特异性标记。通过与固定在硝酸纤维素滤膜上的重组DNA质粒携带的果蝇tRNA基因高效退火，对各个同功受体的水平进行定量。检测了从主要tRNAVal3b基因座缺失的果蝇中分离出的tRNA中tRNAVal3b的水平。结果表明，主要tRNAVal3b基因座的缺失导致tRNAVal3b水平降低约50%，但未产生微小表型；此外，两个基因座缺失的影响大致呈累加效应。
