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通过气相色谱分析细胞脂肪酸作为鉴定医学上重要棒状杆菌的一种工具。

Analysis of cellular fatty acids by gas chromatography as a tool in the identification of medically important coryneform bacteria.

作者信息

Athalye M, Noble W C, Minnikin D E

出版信息

J Appl Bacteriol. 1985 May;58(5):507-12. doi: 10.1111/j.1365-2672.1985.tb01491.x.

DOI:10.1111/j.1365-2672.1985.tb01491.x
PMID:3924876
Abstract

The fatty acid methyl esters of nineteen unidentified pathogenic coryneform bacteria were analysed by gas-liquid chromatography and the resulting profiles were compared with those of type or reference strains of possibly related species, namely Caseobacter polymorphus, Corynebacterium bovis, C. diphtheriae, C. xerosis and Rhodococcus equi. All of the strains had distinct fatty acid profiles but most of them conformed to a general pattern, with high levels of octadecanoic acids and only trace amounts of 10-methyl octadecanoic acid (tuberculostearic acid). These profiles were very similar to those from C. diphtheriae and C. xerosis but could be differentiated from C. bovis, Cas. polymorphus, R. equi and two unidentified pathogenic strains which had significantly higher levels of tuberculostearic acid.

摘要

采用气液色谱法分析了19株未鉴定的致病性棒状杆菌的脂肪酸甲酯,并将所得图谱与可能相关物种的模式菌株或参考菌株的图谱进行了比较,这些相关物种包括多形产碱菌、牛棒状杆菌、白喉棒状杆菌、干燥棒状杆菌和马红球菌。所有菌株都有独特的脂肪酸图谱,但大多数符合一般模式,即十八烷酸含量高,而10-甲基十八烷酸(结核硬脂酸)含量仅为痕量。这些图谱与白喉棒状杆菌和干燥棒状杆菌的图谱非常相似,但可与牛棒状杆菌、多形产碱菌、马红球菌以及另外两株未鉴定的致病性菌株区分开来,后几株菌株的结核硬脂酸含量明显更高。

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