Master Lab for Innovative Application of Nature Products, National Center of Technology Innovation for Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
Henan Engineering Research Center of Food Microbiology, College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471023, China.
Sci Rep. 2024 Sep 10;14(1):21168. doi: 10.1038/s41598-024-72225-1.
Ginsenoside Rb exhibits a wide range of biological activities, and gut microbiota is considered the main metabolic site for Rb. However, the impact of gut microbiota on the pharmacokinetics of Rb are still uncertain. In this study, we investigated the gut microbiome changes and the pharmacokinetics after a 30 d Rb intervention. Results reveal that the systemic exposure and metabolic clearance rate of Rb and Rd were substantially affected after orally supplementing Rb (60 mg/kg) to rats. Significant increase in the relative abundance of Bacteroides cellulosilyticus in gut microbiota and specific glycoside hydrolase (GH) families, such as GH2, GH92, and GH20 were observed based on microbiome and metagenomic analysis. Moreover, a robust association was identified between the pharmacokinetic parameters of Rb and the relative abundance of specific Bacteroides species, and glycoside hydrolase families. Our study demonstrates that Rb administration significantly affects the gut microbiome, revealing a complex relationship between B. cellulosilyticus, key GH families, and Rb pharmacokinetics.
人参皂苷 Rb 具有广泛的生物活性，肠道微生物群被认为是 Rb 的主要代谢部位。然而，肠道微生物群对 Rb 药代动力学的影响仍不确定。在这项研究中，我们研究了 30 天 Rb 干预后肠道微生物组的变化和药代动力学。结果表明，口服补充 Rb（60mg/kg）后，Rb 和 Rd 的全身暴露量和代谢清除率有显著变化。基于微生物组和宏基因组分析，发现肠道微生物群中 Bacteroides cellulosilyticus 的相对丰度显著增加，并且特定的糖苷水解酶（GH）家族，如 GH2、GH92 和 GH20 也显著增加。此外，还鉴定到 Rb 的药代动力学参数与特定的拟杆菌属物种和糖苷水解酶家族的相对丰度之间存在很强的关联。我们的研究表明，Rb 给药显著影响肠道微生物群，揭示了 B. cellulosilyticus、关键 GH 家族和 Rb 药代动力学之间的复杂关系。
