不同抗凝剂对犬常规血浆生化指标的影响。

The effects of different anticoagulants on routine canine plasma biochemistry.

作者信息

Cerón José J, Martínez-Subiela Silvia, Hennemann Carla, Tecles Fernando

机构信息

Clinical Pathology Section, Animal Pathology Department, Veterinary School, University of Murcia, 30100 Espinardo, Murcia, Spain.

出版信息

Vet J. 2004 May;167(3):294-301. doi: 10.1016/j.tvjl.2003.09.009.

DOI:10.1016/j.tvjl.2003.09.009

PMID:15080879

Abstract

The effects of heparin, ethylenediaminetetraacetic acid (EDTA), sodium citrate and sodium fluoride/potassium oxalate on plasma biochemistry results in dogs were studied and compared with serum. Blood specimens from 10 apparently clinical healthy dogs were collected and placed in different tubes containing each anticoagulant tested. Differences in albumin, acetylcholinesterase, ionized calcium and potassium were found between serum and heparinized plasma. Most metabolites and enzymes did not show any variation, but significant decreases in electrolytes, alkaline phosphatase, acetylcholinesterase, bile acids, fructosamine and albumin were found when EDTA was used. Sodium citrate produced a 10-15% decrease in most metabolites and enzymes, possibly due to a sample dilution effect. Sodium fluoride/potassium oxalate produced haemolysis which may have influenced changes in some biochemical parameters.

摘要

研究了肝素、乙二胺四乙酸（EDTA）、柠檬酸钠和氟化钠/草酸钾对犬血浆生化结果的影响，并与血清进行了比较。采集了10只临床健康犬的血样，分别置于含有每种测试抗凝剂的不同试管中。发现血清和肝素化血浆之间的白蛋白、乙酰胆碱酯酶、离子钙和钾存在差异。大多数代谢物和酶没有显示出任何变化，但使用EDTA时，电解质、碱性磷酸酶、乙酰胆碱酯酶、胆汁酸、果糖胺和白蛋白显著降低。柠檬酸钠使大多数代谢物和酶减少了10 - 15%，这可能是由于样本稀释效应。氟化钠/草酸钾导致溶血，这可能影响了一些生化参数的变化。