Wang Wei-Yu, Kong Wei-Liang, Liao Yang-Chun-Zi, Zhu Li-Hua
College of Forestry, Nanjing Forestry University, Nanjing 210037, China.
College of Plant Protection, China Agricultural University, Beijing 100193, China.
J Fungi (Basel). 2022 Sep 28;8(10):1021. doi: 10.3390/jof8101021.
Traditional control methods have drawbacks in controlling Verticillium wilt diseases caused by Kleb.; therefore, an efficient and environmentally friendly strategy for disease control must be identified and the mechanisms determined. In this study, a soil-isolated strain SBB was identified as based on 16S rRNA, , and gene sequences. In vitro, strain SBB had excellent inhibitory effects on , with the highest inhibition rate of 70.94%. Moreover, strain SBB inhibited production of the conidia of and suppressed the production of microsclerotia and melanin. Through gas chromatograph-mass spectrometer analysis, nine compounds were detected from the volatile organic compounds produced by SBB, among which 2-nonanol, 2-heptanone, 6-methyl-2-heptanone, and 2-nonanone could completely inhibit growth. Strain SBB produced cellulase, amylase, protease, and siderophore. During inhibitory action on , strain SBB showed upregulated expression of genes encoding non-volatile inhibitory metabolites, including difficidin, bacilysin, and bacillaene, at 1.923-, 1.848-, and 1.448-fold higher, respectively. Thus, our study proved that strain SBB had an efficient antagonistic effect on , suggesting strain SBB can be used as a potential biological control agent against Verticillium wilt.
传统的防治方法在控制由克莱布氏菌引起的黄萎病方面存在缺陷；因此，必须确定一种高效且环保的病害防治策略并明确其作用机制。在本研究中，基于16S rRNA、[此处原文缺失部分内容]和[此处原文缺失部分内容]基因序列，从土壤中分离出的菌株SBB被鉴定为[此处原文缺失部分内容]。在体外，菌株SBB对[此处原文缺失部分内容]具有优异的抑制作用，最高抑制率达70.94%。此外，菌株SBB抑制了[此处原文缺失部分内容]分生孢子的产生，并抑制了小菌核和黑色素的产生。通过气相色谱 - 质谱分析，从SBB产生的挥发性有机化合物中检测到9种化合物，其中2 - 壬醇、2 - 庚酮、6 - 甲基 - 2 - 庚酮和2 - 壬酮可完全抑制[此处原文缺失部分内容]的生长。菌株SBB产生纤维素酶、淀粉酶、蛋白酶和铁载体。在对[此处原文缺失部分内容]的抑制作用过程中，菌株SBB编码非挥发性抑制性代谢产物（包括艰难梭菌素、芽孢溶素和杆菌烯）的基因表达上调，分别比对照高1.923倍、1.848倍和1.448倍。因此，我们的研究证明菌株SBB对[此处原文缺失部分内容]具有高效拮抗作用，表明菌株SBB可作为防治黄萎病的潜在生物防治剂。
