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健康和腹泻猫肠道菌的流行情况及多样性

Prevalence and diversity of enteric spp. in healthy and diarrheic cats.

作者信息

Ahmadi Reza, Arfaee Farnoosh, Jamshidi Shahram, Mortazavi Pejman, Jamshidian Mahmoud

机构信息

Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Department of Internal Medicine, School of Veterinary Medicine, University of Tehran, Tehran, Iran.

出版信息

Iran J Microbiol. 2024 Aug;16(4):569-575. doi: 10.18502/ijm.v16i4.16317.

Abstract

BACKGROUND AND OBJECTIVES

Helicobacters are gastric and enterohepatic and live in the gut. The role of enterohepatic Helicobacters as intestinal pathogens is uncertain, while stomach Helicobacters are well-known. The prevalence of species in cat feces helps us understand their impact on cat health and human disease transmission. This study used PCR to identify spp. in feces samples from healthy and diarrhoeic cats, independent of the reason. The study also compared intestinal and stomach species.

MATERIALS AND METHODS

PCR analysis was performed on fecal samples from 40 cats, with 20 cats having diarrhea and 20 cats showing no symptoms. The PCR analysis aimed to detect Helicobacter's presence using a method that identifies the bacteria through the 16S rRNA gene.

RESULTS

The diarrhoeic group had a greater prevalence of infection (17:9 ratio), with an overall 65% infection rate detected. Cats that were older than 2 years showed a higher incidence of disease. had the highest occurrence rate (69.2%), followed by and Significantly, and were not reported.

CONCLUSION

was the predominant species found in both healthy and diarrheic cats, indicating the need for more investigation. The detection of the gastric species and further complicates the classification. This highlights the complex nature of infections in cats, emphasizing the need for further investigation to guide the development of preventative measures and treatment techniques for both veterinary and public health purposes.

摘要

背景与目的

螺杆菌存在于胃和肝肠中,寄居于肠道。肝肠螺杆菌作为肠道病原体的作用尚不确定,而胃螺杆菌则广为人知。猫粪便中该菌种的流行情况有助于我们了解它们对猫健康的影响以及对人类疾病传播的作用。本研究使用聚合酶链反应(PCR)来鉴定健康猫和腹泻猫粪便样本中的螺杆菌属菌种,无论腹泻原因如何。该研究还比较了肠道和胃中的螺杆菌菌种。

材料与方法

对40只猫的粪便样本进行PCR分析,其中20只猫患有腹泻,20只猫无症状。PCR分析旨在通过一种利用16S核糖体RNA基因鉴定细菌的方法来检测螺杆菌的存在。

结果

腹泻组的感染率更高(比例为17:9),总体检测到的感染率为65%。2岁以上的猫发病率更高。幽门螺杆菌的发生率最高(69.2%),其次是犬螺杆菌和猫螺杆菌。值得注意的是,未报告发现海尔曼螺杆菌和毕氏螺杆菌。

结论

幽门螺杆菌是在健康猫和腹泻猫中均发现的主要菌种,这表明需要进行更多研究。胃菌种海尔曼螺杆菌和毕氏螺杆菌的检测使分类进一步复杂化。这突出了猫螺杆菌感染的复杂性,强调需要进一步研究以指导兽医和公共卫生目的的预防措施和治疗技术的开发。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52c9/11389771/621339b9d8db/IJM-16-569-g002.jpg

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