Ortega-Nieto Clara, Salta Maria, Noël-Hermes Nanni, Palomo Jose M
Instituto de Catálisis y Petroleoquímica (ICP), CSIC, c/Marie Curie 2, 28049 Madrid, Spain.
Endures B.V., 1781 AT Den Helder, The Netherlands.
Nanomaterials (Basel). 2024 Aug 23;14(17):1376. doi: 10.3390/nano14171376.
In search of new materials that would help to prevent microbiologically influenced corrosion (MIC), we have designed and synthetized six different copper and copper-silver nanoparticle-enzyme hybrids using a mild-conditions method carried out in water and r.t. Characterization analyses exhibited the presence of small crystalline nanoparticles with diameters from 2 to 20 nm. X-ray diffraction determined that the Cu hybrids were composed of different copper species, depending on the synthetic protocol used, while the Cu-Ag hybrids were mainly composed of copper and silver phosphate metallic species. Then, the bacterial viability of three MIC-relevant enrichments, sulfate-reducing bacteria (SRB), slime-forming bacteria (SFB), and acid-producing bacteria (APB), was studied in the presence of the bionanohybrids. The results demonstrated a notable effect of all bionanohybrids against SRB, one of the most prominent bacteria associated with MIC. In particular, and - showed a reduction in bacterial cells of 94% and 98% after 48 h, respectively, at a concentration of 100 ppm. They also exhibited high efficiencies against SFB, with - and - hybrids being the best, with bacterial reduction percentages of 98% after 45 h of exposition at a concentration of 100 ppm. However, in the case of APB, the effect of the hybrids was lost due to the low pH level generated during the experiment. Finally, the capacity of and - to inhibit the adhesion of SRB to the surface of carbon steel coupons was evaluated. Fluorescence imaging of the surface of the coupons at 24 h demonstrated that the presence of the hybrids inhibited the growth of SRB, obtaining a maximum reduction of 98% with . Overall, the results of this study demonstrate that these novel nanomaterials have a wide-range antibacterial effect and may have a promising future in the prevention and treatment of MIC.
为了寻找有助于防止微生物影响腐蚀（MIC）的新材料，我们采用在水和室温下进行的温和条件方法，设计并合成了六种不同的铜和铜 - 银纳米颗粒 - 酶杂化物。表征分析表明存在直径为2至20nm的小结晶纳米颗粒。X射线衍射确定，根据所使用的合成方案，铜杂化物由不同的铜物种组成，而铜 - 银杂化物主要由铜和磷酸银金属物种组成。然后，在生物纳米杂化物存在的情况下，研究了三种与MIC相关的富集菌，即硫酸盐还原菌（SRB）、产粘菌（SFB）和产酸菌（APB）的细菌活力。结果表明，所有生物纳米杂化物对SRB均有显著效果，SRB是与MIC相关的最主要细菌之一。特别是，在100 ppm浓度下，48小时后，[具体物质1]和[具体物质2]分别使细菌细胞减少了94%和98%。它们对SFB也表现出高效性，[具体物质3]和[具体物质4]杂化物效果最佳，在100 ppm浓度下暴露45小时后细菌减少率为98%。然而，对于APB，由于实验过程中产生的低pH值，杂化物的效果消失。最后，评估了[具体物质5]和[具体物质6]抑制SRB粘附到碳钢试片表面的能力。24小时时试片表面的荧光成像表明，杂化物的存在抑制了SRB的生长，[具体物质7]使SRB生长减少最多达98%。总体而言，本研究结果表明，这些新型纳米材料具有广泛的抗菌作用，在MIC的预防和治疗方面可能具有广阔的前景。
