College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
College of Grassland Resources, Southwest Minzu University, Chengdu 610041, China.
Int J Mol Sci. 2024 Aug 29;25(17):9383. doi: 10.3390/ijms25179383.
The alternative oxidase (AOX), a common terminal oxidase in the electron transfer chain (ETC) of plants, plays a crucial role in stress resilience and plant growth and development. Oat (), an important crop with high nutritional value, has not been comprehensively studied regarding the AsAOX gene family. Therefore, this study explored the responses and potential functions of the AsAOX gene family to various abiotic stresses and their potential evolutionary pathways. Additionally, we conducted a genome-wide analysis to explore the evolutionary conservation and divergence of AOX gene families among three species (, , ) and four species (, , , and ). We identified 12 AsAOX, 9 AiAOX, and 4 AlAOX gene family members. Phylogenetic, motif, domain, gene structure, and selective pressure analyses revealed that most AsAOXs, AiAOXs, and AlAOXs are evolutionarily conserved. We also identified 16 AsAOX segmental duplication pairs, suggesting that segmental duplication may have contributed to the expansion of the AsAOX gene family, potentially preserving these genes through subfunctionalization. Chromosome polyploidization, gene structural variations, and gene fragment recombination likely contributed to the evolution and expansion of the AsAOX gene family as well. Additionally, we hypothesize that may have potential function in resisting wounding and heat stresses, while could be specifically involved in mitigating wounding stress. might contribute to resistance against chromium and waterlogging stresses. may have potential fuction in mitigating ABA-mediated stress. and are most likely to have potential function in mitigating salt and drought stresses, respectively. This study elucidates the potential evolutionary pathways of the AsAOXs gene family, explores their responses and potential functions to various abiotic stresses, identifies potential candidate genes for future functional studies, and facilitates molecular breeding applications in .
交替氧化酶（AOX）是植物电子传递链（ETC）中的一种常见末端氧化酶，在应激抗性和植物生长发育中起着至关重要的作用。燕麦（）是一种具有高营养价值的重要作物，但关于其 AsAOX 基因家族的研究还不够全面。因此，本研究探讨了 AsAOX 基因家族对各种非生物胁迫的响应和潜在功能，以及它们潜在的进化途径。此外，我们进行了全基因组分析，以探讨三种（、和）和四种（、、和）物种中 AOX 基因家族的进化保守性和分化。我们鉴定了 12 个 AsAOX、9 个 AiAOX 和 4 个 AlAOX 基因家族成员。系统发育、基序、结构域、基因结构和选择压力分析表明，大多数 AsAOX、AiAOX 和 AlAOX 是进化保守的。我们还鉴定了 16 个 AsAOX 片段重复对，表明片段重复可能有助于 AsAOX 基因家族的扩张，可能通过亚功能化来保存这些基因。染色体多倍化、基因结构变异和基因片段重组也可能对 AsAOX 基因家族的进化和扩张做出贡献。此外，我们假设可能在抵抗创伤和热应激方面具有潜在功能，而可能特别参与减轻创伤应激。可能有助于抵抗铬和水淹胁迫。可能具有缓解 ABA 介导的胁迫的潜在功能。和可能分别具有减轻盐和干旱胁迫的潜在功能。本研究阐明了 AsAOX 基因家族的潜在进化途径，探讨了它们对各种非生物胁迫的响应和潜在功能，鉴定了未来功能研究的潜在候选基因，并促进了燕麦分子育种应用。
