Department of Pathology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
Int J Mol Sci. 2024 Aug 31;25(17):9482. doi: 10.3390/ijms25179482.
Breast cancer is the most prevalent malignant tumor among women with high heterogeneity. Traditional techniques frequently struggle to comprehensively capture the intricacy and variety of cellular states and interactions within breast cancer. As global precision medicine rapidly advances, single-cell RNA sequencing (scRNA-seq) has become a highly effective technique, revolutionizing breast cancer research by offering unprecedented insights into the cellular heterogeneity and complexity of breast cancer. This cutting-edge technology facilitates the analysis of gene expression profiles at the single-cell level, uncovering diverse cell types and states within the tumor microenvironment. By dissecting the cellular composition and transcriptional signatures of breast cancer cells, scRNA-seq provides new perspectives for understanding the mechanisms behind tumor therapy, drug resistance and metastasis in breast cancer. In this review, we summarized the working principle and workflow of scRNA-seq and emphasized the major applications and discoveries of scRNA-seq in breast cancer research, highlighting its impact on our comprehension of breast cancer biology and its potential for guiding personalized treatment strategies.
乳腺癌是女性中最常见的恶性肿瘤，具有高度异质性。传统技术常常难以全面捕捉乳腺癌中细胞状态和相互作用的复杂性和多样性。随着全球精准医学的快速发展，单细胞 RNA 测序（scRNA-seq）已成为一种非常有效的技术，通过提供对乳腺癌细胞异质性和复杂性的前所未有的见解，彻底改变了乳腺癌的研究。这项前沿技术促进了在单细胞水平上分析基因表达谱，揭示了肿瘤微环境中多样化的细胞类型和状态。通过剖析乳腺癌细胞的细胞组成和转录特征，scRNA-seq 为理解肿瘤治疗、乳腺癌耐药和转移背后的机制提供了新的视角。在这篇综述中，我们总结了 scRNA-seq 的工作原理和工作流程，并强调了 scRNA-seq 在乳腺癌研究中的主要应用和发现，突出了其对我们理解乳腺癌生物学的影响及其指导个性化治疗策略的潜力。
