Department of Otolaryngology, Head and Neck Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang University, Nanchang, 330006, Jiangxi, People's Republic of China.
Mol Biotechnol. 2024 Jul;66(7):1497-1519. doi: 10.1007/s12033-023-00777-0. Epub 2023 Jun 15.
Single-cell RNA-seq (scRNA-seq) is a revolutionary technology that allows for the genomic investigation of individual cells in a population, allowing for the discovery of unusual cells associated with cancer and metastasis. ScRNA-seq has been used to discover different types of cancers with poor prognosis and medication resistance such as lung cancer, breast cancer, ovarian cancer, and gastric cancer. Besides, scRNA-seq is a promising method that helps us comprehend the biological features and dynamics of cell development, as well as other disorders. This review gives a concise summary of current scRNA-seq technology. We also explain the main technological steps involved in implementing the technology. We highlight the present applications of scRNA-seq in cancer research, including tumor heterogeneity analysis in lung cancer, breast cancer, and ovarian cancer. In addition, this review elucidates potential applications of scRNA-seq in lineage tracing, personalized medicine, illness prediction, and disease diagnosis, which reveals that scRNA-seq facilitates these events by producing genetic variations on the single-cell level.
单细胞 RNA 测序（scRNA-seq）是一项革命性的技术，可对群体中的单个细胞进行基因组研究，从而发现与癌症和转移相关的异常细胞。scRNA-seq 已被用于发现预后不良和耐药的不同类型的癌症，如肺癌、乳腺癌、卵巢癌和胃癌。此外，scRNA-seq 是一种很有前途的方法，可以帮助我们理解细胞发育的生物学特征和动态，以及其他疾病。本综述简要总结了当前的 scRNA-seq 技术。我们还解释了实施该技术所涉及的主要技术步骤。我们重点介绍了 scRNA-seq 在癌症研究中的当前应用，包括肺癌、乳腺癌和卵巢癌中的肿瘤异质性分析。此外，本综述阐明了 scRNA-seq 在谱系追踪、个性化医疗、疾病预测和疾病诊断中的潜在应用，这表明 scRNA-seq 通过在单细胞水平上产生遗传变异来促进这些事件。
