Department of Biomaterials Science, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
J Dent. 2018 Mar;70:92-96. doi: 10.1016/j.jdent.2017.12.017. Epub 2017 Dec 30.
Resin composites containing surface pre-reacted glass-ionomer (S-PRG) fillers have been reported to inhibit Streptococcus mutans growth on their surfaces, and their inhibitory effects were attributed to BO and F ions. The aim of this study was to evaluate S. mutans acid production through glucose metabolism on resin composite containing S-PRG fillers and assess inhibitory effects of BO and F on S. mutans metabolic activities.
The pH change through S. mutans acid production on experimental resin composite was periodically measured after the addition of glucose. Inhibitory effects of BO or F solutions on S. mutans metabolism were evaluated by XTT assays and measurement of the acid production rate.
The pH of experimental resin containing S-PRG fillers was significantly higher than that of control resin containing silica fillers (p < 0.05). OD values by XTT assays and S. mutans acid production rates significantly decreased in the presence of BO and F compared with the absence of these ions (p < 0.05).
pH reduction by S. mutans acid production was inhibited on resin composite containing S-PRG fillers. Moreover, S. mutans glucose metabolism and acid production were inhibited in the presence of low concentrations of BO or F.
BO or F released from resin composite containing S-PRG fillers exhibits inhibitory effects on S. mutans metabolism at concentrations lower than those which inhibit bacterial growth.
含有表面预反应玻璃离子（S-PRG）填料的树脂复合材料已被报道能够抑制其表面变形链球菌的生长，其抑制作用归因于 BO 和 F 离子。本研究旨在评估含有 S-PRG 填料的树脂复合材料中 S. mutans 通过葡萄糖代谢产生的酸，并评估 BO 和 F 对 S. mutans 代谢活性的抑制作用。
在添加葡萄糖后，定期测量实验性树脂复合材料中 S. mutans 产酸导致的 pH 变化。通过 XTT 测定法和产酸速率测量来评估 BO 或 F 溶液对 S. mutans 代谢的抑制作用。
含有 S-PRG 填料的实验性树脂的 pH 值明显高于含有二氧化硅填料的对照树脂（p<0.05）。与不存在这些离子相比，存在 BO 和 F 时 XTT 测定法的 OD 值和 S. mutans 产酸速率显著降低（p<0.05）。
含有 S-PRG 填料的树脂复合材料中 S. mutans 产酸导致的 pH 值降低受到抑制。此外，在低浓度的 BO 或 F 存在下，S. mutans 葡萄糖代谢和产酸受到抑制。
含有 S-PRG 填料的树脂复合材料中释放的 BO 或 F 在抑制细菌生长的浓度以下对 S. mutans 代谢具有抑制作用。
