Yang Mengqi, Min Tian, Manda Teja, Yang Liming, Hwarari Delight
State Key Laboratory of Tree Genetics and Breeding, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China.
Plants (Basel). 2024 Aug 27;13(17):2387. doi: 10.3390/plants13172387.
The impact of global warming is increasing and thus exacerbating environmental stresses that affect plant yield and distribution, including the Lindl (Loquat tree). , a member of the Rosaceae family, is valued not only for its nutritious fruit but also for its medicinal purposes, landscape uses, and other pharmacological benefits. Nonetheless, the productivity of has raised a lot of concern in the wake of adverse environmental conditions. Understanding the characteristics of the gene family in loquat is crucial, as these genes play vital roles in plant stress responses. In this study, 283 genes were identified in the genome of that were randomly positioned on 17 chromosomes and 24 contigs. The 283 EjLRR-RLK proteins clustered into 21 classes and subclasses in the phylogenetic analysis based on domain and protein arrangements. Further explorations in the promoter regions of the genes showed an abundance of cis-regulatory elements that functioned in growth and development, phytohormone, and biotic and abiotic responses. Most cis-elements were present in the biotic and abiotic responses suggesting that the genes are invested in regulating both biotic and abiotic stresses. Additional investigations into the responses of genes to abiotic stress using the RT-qPCR revealed that genes respond to abiotic stress, especially heat and salt stresses. Particularly, and exhibited constant upregulation in all stresses analyzed, indicating that these may take an active role in regulating abiotic stresses. Our findings suggest the pivotal functions of genes although additional research is still required. This research aims to provide useful information relating to the characterization of genes and their responses to environmental stresses, establishing a concrete base for the following research.
全球变暖的影响日益增加，从而加剧了影响植物产量和分布的环境压力，包括枇杷（蔷薇科的一种）。枇杷不仅因其营养丰富的果实而受到重视，还因其药用价值、景观用途和其他药理益处而受到关注。尽管如此，在不利的环境条件下，枇杷的生产力引发了诸多关注。了解枇杷中该基因家族的特征至关重要，因为这些基因在植物应激反应中发挥着关键作用。在本研究中，在枇杷基因组中鉴定出283个该基因，它们随机定位在17条染色体和24个重叠群上。基于结构域和蛋白质排列的系统发育分析中，283个EjLRR - RLK蛋白聚为21个类别和亚类。对该基因启动子区域的进一步探索表明，存在大量在生长发育、植物激素以及生物和非生物反应中起作用的顺式调控元件。大多数顺式元件存在于生物和非生物反应中，这表明该基因参与调节生物和非生物胁迫。使用RT - qPCR对该基因对非生物胁迫的反应进行的额外研究表明，该基因对非生物胁迫有反应，尤其是热胁迫和盐胁迫。特别是，在所有分析的胁迫中，该基因持续上调，表明这些基因可能在调节非生物胁迫中发挥积极作用。我们的研究结果表明了该基因的关键功能，尽管仍需要更多研究。本研究旨在提供与该基因的特征及其对环境胁迫的反应相关的有用信息，为后续研究奠定坚实基础。
