Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.
College of Animal Science, Xichang University, Xichang 615013, China.
Molecules. 2024 Sep 4;29(17):4205. doi: 10.3390/molecules29174205.
Methicillin-resistant (MRSA) is a highly threatening foodborne pathogen capable of causing severe organ and life-threatening diseases. Over the past years, various commercial antibiotics have been used to treat MRSA infections. However, these commercial antibiotics have not yielded efficient results and also cause other side effects; therefore, there is a need for the development of effective alternatives to replace these commercial antibiotics. Suberanilic acid, an amide alkaloid obtained from the endophytic fungus DCL44, has been identified as a significant antimicrobial agent. However, its antibiotic properties on multi-drug-resistant bacteria such as MRSA have not been fully explored. Therefore, to investigate the potential antimicrobial mechanism of suberanilic acid against MRSA, a quantitative proteomics approach using tandem mass tagging (TMT) was used. The results obtained in the study revealed that suberanilic acid targets multiple pathways in MRSA, including disruption of ribosome synthesis, inhibition of membrane translocation for nutrient uptake (ABC transporter system), and causing dysregulation of carbohydrate and amino acid energy metabolism. These results provide new insights into the mechanism of action of suberanilic acid against MRSA and offer technical support and a theoretical basis for the development of novel food antimicrobial agents derived from endophytic fungal origin.
耐甲氧西林金黄色葡萄球菌（MRSA）是一种具有高度威胁性的食源性病原体，能够导致严重的器官和危及生命的疾病。在过去的几年中，已经使用了各种商业抗生素来治疗 MRSA 感染。然而，这些商业抗生素并没有产生有效的效果，并且还会引起其他副作用；因此，需要开发有效的替代品来替代这些商业抗生素。从内生真菌 DCL44 中获得的酰胺生物碱琥珀酰亚胺酸已被确定为一种重要的抗菌剂。然而，其对多药耐药菌如 MRSA 的抗生素特性尚未得到充分探索。因此，为了研究琥珀酰亚胺酸对 MRSA 的潜在抗菌机制，使用串联质量标记（TMT）的定量蛋白质组学方法进行了研究。研究结果表明，琥珀酰亚胺酸靶向 MRSA 中的多个途径，包括破坏核糖体合成、抑制营养摄取的膜易位（ABC 转运系统），以及导致碳水化合物和氨基酸能量代谢失调。这些结果为琥珀酰亚胺酸对 MRSA 的作用机制提供了新的见解，并为开发源自内生真菌的新型食品抗菌剂提供了技术支持和理论依据。
