Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt.
Department of Zoology, Faculty of Science, Damanhur University, Damanhur, Egypt.
Sci Rep. 2024 Sep 14;14(1):21519. doi: 10.1038/s41598-024-70060-y.
The common housefly, Musca domestica, known for transmitting over 100 infections, was studied using green-synthesized Cadmium Sulfide nanoparticles (CdS NPs) from Agaricus bisporus. These CdS NPs were tested on third-instar larvae under laboratory conditions using dipping and feeding methods with concentrations (75, 100, 125, 150, 175, and 200 µg/mL). The toxicity, measured by LC50, was found to be 138 µg/mL for dipping treatment and 123 µg/mL for feeding treatment. Analysis with an energy-dispersive X-ray microanalyzer confirmed Cd accumulation in the larval midgut, indicating penetration of CdS NPs into the organism, which may potentially increase their toxicity. CdS NPs caused disruptions in Heat Shock Protein 70, cell apoptosis, and various biochemical components. Scanning electron microscopy revealed morphological abnormalities in larvae, pupae, and adults exposed to CdS NPs. Ultrastructural examination showed significant midgut tissue abnormalities in larvae treated with 123 µg/mL of CdS NPs. Our study demonstrated that green-synthesized CdS NPs from A. bisporus can effectively control the development of M. domestica larvae.
常见家蝇（Musca domestica）以传播超过 100 种感染而闻名，本研究使用双孢蘑菇（Agaricus bisporus）合成的绿色硫化镉纳米粒子（CdS NPs）对其进行了研究。在实验室条件下，使用浸渍和喂养方法，将这些 CdS NPs 以浓度（75、100、125、150、175 和 200µg/mL）施用于三龄幼虫。通过 LC50 测定，发现浸渍处理的毒性为 138µg/mL，而喂养处理的毒性为 123µg/mL。用能量色散 X 射线微分析仪进行分析证实了 Cd 在幼虫中肠的积累，这表明 CdS NPs 穿透了生物体，这可能会增加它们的毒性。CdS NPs 导致热休克蛋白 70 中断、细胞凋亡和各种生化成分的改变。扫描电子显微镜显示暴露于 CdS NPs 的幼虫、蛹和成虫出现形态异常。超微结构检查显示，用 123µg/mL 的 CdS NPs 处理的幼虫的中肠组织有明显异常。本研究表明，双孢蘑菇合成的绿色硫化镉纳米粒子可以有效控制家蝇幼虫的发育。
