Mukundan Divya, Jeevanandan Ganesh
Pediatric and Preventive Dentistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Pediatric and Preventive Dentisty, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Cureus. 2024 Aug 16;16(8):e66999. doi: 10.7759/cureus.66999. eCollection 2024 Aug.
Introduction The endodontic treatment of primary teeth presents considerable complications due to their distinct anatomical properties. In order to achieve a successful endodontic treatment, certain factors must be assessed. These factors include a precise diagnosis, thorough cleaning, and a reliable disinfection protocol. Although sodium hypochlorite (NaOCl) has been effective as an irrigation agent in primary teeth, it is important to recognize that higher concentrations of NaOCl might possibly inflict toxic harm on the periapical environment if they penetrate the tooth's apical foramina. Since primary teeth are important, pediatric dentists must choose an appropriate NaOCl concentration for root canal irrigation, as higher concentrations can be toxic. Thus, the current investigation examined the cytotoxicity of two different NaOCl concentrations at various volumes. Methods To evaluate the cytotoxicity potential, a culture of nauplii (brine shrimp) was prepared and subjected to testing. For the test, 5, 10, 20, and 40 µL of 1% and 3% NaOCl were added to the brine shrimp culture at different concentrations, and saline was used as a control. After a span of 24 hours, the total number of alive nauplii was duly noted. Results After 24 hours, nauplii showed no mortality in the control group. For 1% NaOCl, mortality ranged from 10% to 20% across volumes, with no significant differences (p = 0.193). In contrast, 3% NaOCl caused significantly higher mortality: 20% at 5 µL, 30% at 10 and 20 µL, and 60% at 40 µL (p = 0.007). Tukey's analysis revealed no significant differences for 1% NaOCl (p > 0.05) but significant differences for 3% NaOCl at 40 µL (p < 0.05). Conclusion Based on the results of the present study, it was observed that a 1% NaOCl solution exhibited a lower level of toxicity in comparison to a 3% NaOCl solution. These findings highlight the importance of using lower concentrations of NaOCl for endodontic irrigation in pediatric dentistry to reduce the risk of tissue damage and ensure safer outcomes for young patients.
引言 由于乳牙独特的解剖结构，乳牙的牙髓治疗存在相当多的并发症。为了实现成功的牙髓治疗，必须评估某些因素。这些因素包括准确的诊断、彻底的清洁和可靠的消毒方案。尽管次氯酸钠（NaOCl）作为乳牙根管冲洗剂已被证明有效，但必须认识到，如果高浓度的NaOCl穿透牙齿的根尖孔，可能会对根尖周环境造成毒性损害。由于乳牙很重要，儿童牙医必须为根管冲洗选择合适的NaOCl浓度，因为高浓度可能有毒。因此，本研究检测了两种不同浓度的NaOCl在不同体积下的细胞毒性。方法 为了评估细胞毒性潜力，制备了无节幼体（卤虫）培养物并进行测试。在测试中，将5、10、20和40微升1%和3%的NaOCl以不同浓度添加到卤虫培养物中，并用生理盐水作为对照。24小时后，记录存活无节幼体的总数。结果 24小时后，对照组无节幼体无死亡。对于1%的NaOCl，不同体积下的死亡率在10%至20%之间，无显著差异（p = 0.193）。相比之下，3%的NaOCl导致的死亡率显著更高：5微升时为20%，10和20微升时为30%，40微升时为60%（p = 0.007）。Tukey分析显示1%的NaOCl无显著差异（p > 0.05），但3%的NaOCl在40微升时有显著差异（p < 0.05）。结论 根据本研究结果，观察到1%的NaOCl溶液与3%的NaOCl溶液相比毒性较低。这些发现凸显了在儿童牙科牙髓冲洗中使用较低浓度NaOCl以降低组织损伤风险并确保年轻患者获得更安全治疗结果的重要性。
