Mohan Tamilselvan, Gürer Fazilet, Bračič Doris, Lackner Florian, Nagaraj Chandran, Maver Uroš, Gradišnik Lidija, Finšgar Matjaž, Kargl Rupert, Kleinschek Karin Stana
Graz University of Technology, Institute of Chemistry and Technology of Biobased System, Stremayrgasse 9, 8010 Graz, Austria.
University of Maribor, Faculty of Mechanical Engineering, Laboratory for Characterisation and Processing of Polymers, Smetanova ulica17, 2000 Maribor, Slovenia.
Biomacromolecules. 2025 Mar 10;26(3):1771-1787. doi: 10.1021/acs.biomac.4c01559. Epub 2025 Feb 23.
This study enhances the bioactivity of polycaprolactone (PCL) scaffolds for tissue engineering by functionalizing them with oxidized hyaluronic acid glycine-peptide conjugates to improve endothelial cell adhesion and growth. Hyaluronic acid was conjugated with a glycine-peptide to create a bioactive interface on PCL (static water contact angle, SCA(HO): 98°). The scaffolds were fabricated using a melt extrusion 3D printing technique. The HA-glycine peptide conjugates were oxidized and immobilized on aminolyzed PCL via Schiff-base chemistry, introducing hydrophilicity (SCA(HO): 21°), multiple functional groups, and a negative zeta potential (-12.04 mV at pH 7.4). A quartz crystal microbalance confirmed chemical conjugation and quantified the mass (8.5-10.3 mg m) of oxidized HA-glycine on PCL. The functionalized scaffolds showed enhanced swelling, improved mechanical properties (2-fold increase in strength, from 26 to 51 MPa), and maintained integrity during degradation. In-vitro experiments demonstrated improved endothelial cell adhesion, proliferation and viability, suggesting the potential for vascularized tissue constructs.
本研究通过用氧化透明质酸甘氨酸 - 肽缀合物对聚己内酯（PCL）支架进行功能化处理，以增强其用于组织工程的生物活性，从而改善内皮细胞的粘附和生长。透明质酸与甘氨酸 - 肽缀合，在PCL上创建一个生物活性界面（静态水接触角，SCA(HO)：98°）。支架采用熔融挤出3D打印技术制造。HA - 甘氨酸肽缀合物经氧化后通过席夫碱化学固定在氨解的PCL上，引入亲水性（SCA(HO)：21°）、多个官能团和负的zeta电位（pH 7.4时为 - 12.04 mV）。石英晶体微天平证实了化学缀合并量化了PCL上氧化HA - 甘氨酸的质量（8.5 - 10.3 mg m）。功能化支架显示出增强的溶胀性、改善的机械性能（强度增加2倍，从26 MPa增至51 MPa），并在降解过程中保持完整性。体外实验表明内皮细胞的粘附、增殖和活力得到改善，表明其在血管化组织构建方面具有潜力。
