Debnath Sandip, Mohanta Deepika, Perveen Kahkashan, Husain Fohad Mabood, Kesari Kavindra Kumar, Ashraf Mohd Shaikhul, Mukerjee Nobendu, Rahin Saima Ahmed
Department of Genetics and Plant Breeding, Institute of Agriculture, Visva-Bharati University, Sriniketan 731236, West Bengal, India.
Department of Botany & Microbiology, College of Science, King Saud University, Riyadh-11495, Saudi Arabia.
Contrast Media Mol Imaging. 2022 Aug 29;2022:9289007. doi: 10.1155/2022/9289007. eCollection 2022.
A series of multidrug extransporters known as the multidrug and potentially toxic extrusion (MATE) genes are found in all living things and are crucial for the removal of heavy metal ions, metalloids, exogenous xenobiotics, endogenous secondary metabolites, and other toxic substances from the cells. However, there has only been a small amount of them in silico analysis of the MATE family of genes in plant species. In the current study, the MATE gene family was characterized in silico where two families and seven subfamilies based on their evolutionary relationships were proposed. Plant breeders may use TraesCS1D02G030400, TraesCS4B02G244400, and TraesCS1A02G029900 genes for marker-assisted or transgenic breeding to develop novel cultivars since these genes have been hypothesized from protein-protein interaction study to play a critical role in the transport of toxic chemicals across cells. The exon number varies from 01 to 14. One exon has TraesCS1A02G188100, TraesCS5B02G562500, TraesCS6A02G256400, and TraesCS6D02G384300 genes, while 14 exons have only two genes that are TraesCS6A02G418800 and TraesCS6D02G407900. Biological stress (infestations of disease) affects the expression of most of the MATE genes, with the gene TraesCS5D02G355500 having the highest expression level in the wheat expression browser tool. Using the Grain interpretation search engine tool, it is found that the vast bulk of MATE genes are voiced throughout biotic environmental stresses caused by disease pests, with the genotype TraesCS5B02G326600.1 from family 1 exhibiting the greatest level of expression throughout head blight infection by after 4 days of infection. The researchers constructed 39 ternary plots, each with a distinct degree of expression under biotic and abiotic stress settings, and observed that 44% of the triplets have imbalanced outputs (extreme values) due to their higher tissue specificity and increased intensity.
一系列被称为多药及潜在毒性外排（MATE）基因的多药外排转运蛋白存在于所有生物中，对于从细胞中清除重金属离子、类金属、外源性异种生物、内源性次生代谢产物及其他有毒物质至关重要。然而，对植物物种中MATE基因家族的计算机分析却很少。在本研究中，对MATE基因家族进行了计算机表征，基于进化关系提出了两个家族和七个亚家族。植物育种者可利用TraesCS1D02G030400、TraesCS4B02G244400和TraesCS1A02G029900基因进行标记辅助育种或转基因育种以培育新品种，因为从蛋白质-蛋白质相互作用研究推测这些基因在有毒化学物质跨细胞转运中起关键作用。外显子数量从01到14不等。一个外显子的有TraesCS1A02G188100、TraesCS5B02G562500、TraesCS6A02G256400和TraesCS6D02G384300基因，而14个外显子的只有两个基因，即TraesCS6A02G418800和TraesCS6D02G407900。生物胁迫（病害侵染）会影响大多数MATE基因的表达，在小麦表达浏览器工具中TraesCS5D02G355500基因的表达水平最高。使用谷物解释搜索引擎工具发现，在由病虫害引起的生物环境胁迫过程中，绝大多数MATE基因都有表达，来自家族1的基因型TraesCS5B02G326600.1在感染4天后的赤霉病感染过程中表达水平最高。研究人员构建了39个三元图，每个三元图在生物和非生物胁迫条件下具有不同程度的表达，并观察到44%的三元组由于其较高的组织特异性和增强的强度而具有不平衡的输出（极值）。
