水稻中砷诱导的多药和有毒化合物排出（MATE）基因功能联系的计算机模拟分析
In silico analysis of functional linkage among arsenic induced MATE genes in rice.
作者信息
Seth Snigdhamayee, Debnath Sandip, Chakraborty N R
机构信息
Department of Genetics & Plant Breeding, Palli Siksha Bhavana (Institute of Agriculture), Visva-Bharati, Sriniketan, 731236, India.
出版信息
Biotechnol Rep (Amst). 2019 Oct 24;26:e00390. doi: 10.1016/j.btre.2019.e00390. eCollection 2020 Jun.
MATE genes play an important role in cellular detoxification processes. Nine MATE genes were identified by a transcriptomics study previously. Candidate gene prioritization was done where 29 new genes were found to interact with 09 guide genes. Therefore, a total of 38 genes were analyzed here to predict a concise model by gene prioritization study. Those genes were analyzed further in Rice Interactions Viewer programme, and based on high ICV, 10 new genes were found to interact among themselves at protein level. Surprisingly, only 05 genes were found to play a key role at protein level. These 15 genes were analyzed for their interaction with soil available inorganic arsenic species. Maximum expression levels were found mostly at young inflorescence and seed development stage for those genes. So, these genes may have a direct role in arsenic sequestration from cells and thereby providing safety to the developing embryo within the seed.
多药和有毒化合物排出（MATE）基因在细胞解毒过程中发挥着重要作用。此前通过转录组学研究鉴定出了9个MATE基因。进行了候选基因优先级排序，发现有29个新基因与9个指导基因相互作用。因此，在此共分析了38个基因，通过基因优先级排序研究预测一个简洁的模型。这些基因在水稻相互作用查看器程序中进一步分析，基于高互作系数值（ICV），发现有10个新基因在蛋白质水平上相互作用。令人惊讶的是，仅发现5个基因在蛋白质水平上起关键作用。分析了这15个基因与土壤中可利用无机砷物种的相互作用。这些基因的最高表达水平大多出现在幼嫩花序和种子发育阶段。所以，这些基因可能在从细胞中螯合砷方面具有直接作用，从而为种子内发育的胚提供安全保障。
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