Hu Ping, Song Puwen, Xu Jun, Wei Qichao, Tao Ye, Ren Yueming, Yu Yongang, Li Dongxiao, Hu Haiyan, Li Chengwei
Henan Engineering Research Center of Crop Genome Editing, Henan International Joint Laboratory of Plant Genetic Improvement and Soil Remediation, College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, China.
School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, China.
Front Plant Sci. 2022 Feb 10;13:847087. doi: 10.3389/fpls.2022.847087. eCollection 2022.
Serine hydroxymethyltransferase () plays a pivotal role in cellular one-carbon, photorespiration pathways and it influences the resistance to biotic and abiotic stresses. However, the function of SHMT proteins in wheat remains largely unexplored. In the present study, genes in five species, , and four dicotyledon species were identified based on whole genome information. The origin history of the target gene was traced by micro-collinearity analysis. Gene expression patterns of in different tissues, various biotic stresses, exogenous hormones, and two biotic stresses were determined by Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The function of the selected was studied by barley stripe mosaic virus-induced gene silencing in common wheat Bainong207. A total of 64 members were identified and further classified into two main classes based on the structure of SHMT proteins. The gene structure and motif composition analyses revealed that kept relatively conserved within the same subclasses. Interestingly, there was a gene, , on chromosome 7B of , but there was no gene on chromosome 7 of other analyzed species; had fewer exons and conserved motifs than the genes in the same subclass, suggesting that the gene of has a notable evolutionary progress. The micro-collinearity relationship showed that no homologs of and its two neighboring genes were found in the collinearity region of , and there were 27 genes inserted into the collinearity region of . . Furthermore, qRT-PCR results showed that was responsive to abiotic stresses (NaCl and cold), abscisic acid, methyl jasmonate, and hydrogen peroxide. Significantly, upon infection, the expression of was highly upregulated in resistant cultivar Sumai3. More importantly, silencing of compromises Fusarium head blight resistance in common wheat Bainong207. Our new findings suggest that the gene in wheat plays an important role in resistance to Fusarium head blight. This provides a valuable reference for further study on the function of this gene family.
丝氨酸羟甲基转移酶（SHMT）在细胞一碳代谢、光呼吸途径中起关键作用，并且影响对生物和非生物胁迫的抗性。然而，SHMT蛋白在小麦中的功能在很大程度上仍未被探索。在本研究中，基于全基因组信息鉴定了5种单子叶植物（小麦、大麦、水稻、玉米和高粱）和4种双子叶植物中的SHMT基因。通过微共线性分析追溯目标基因的起源历史。采用实时定量逆转录聚合酶链反应（qRT-PCR）测定SHMT在不同组织、各种生物胁迫、外源激素以及两种生物胁迫下的基因表达模式。通过大麦条纹花叶病毒诱导的基因沉默在普通小麦百农207中研究所选SHMT的功能。共鉴定出64个SHMT成员，并根据SHMT蛋白的结构进一步分为两个主要类别。基因结构和基序组成分析表明，同一亚类内的SHMT相对保守。有趣的是，在小麦7B染色体上有一个TaSHMT7B基因，但在其他分析的单子叶植物物种的7号染色体上没有SHMT基因；TaSHMT7B的外显子和保守基序比同一亚类中的基因少，表明TaSHMT7B基因有显著的进化进程。微共线性关系表明，在水稻的共线性区域中未发现TaSHMT7B及其两个相邻基因的同源物，并且有27个基因插入到水稻的共线性区域。此外，qRT-PCR结果表明，TaSHMT对非生物胁迫（NaCl和低温）、脱落酸、茉莉酸甲酯和过氧化氢有响应。值得注意的是，在赤霉病菌感染后，TaSHMT在抗病品种苏麦3号中高度上调表达。更重要的是，TaSHMT的沉默削弱了普通小麦百农207对赤霉病的抗性。我们的新发现表明，小麦中的TaSHMT基因在抗赤霉病中起重要作用。这为进一步研究该基因家族的功能提供了有价值的参考。
