Chen Qinfang, Wang Linna, Liu Di, Ma Sirui, Dai Yangshuo, Zhang Xue, Wang Yuxin, Hu Tian, Xiao Ming, Zhou Ying, Qi Hua, Xiao Shi, Yu Lujun
State Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Plants (Basel). 2020 Oct 27;9(11):1448. doi: 10.3390/plants9111448.
Multidrug and Toxic Compound Extrusion (MATE) proteins are essential transporters that extrude metabolites and participate in plant development and the detoxification of toxins. Little is known about the gene family in the Solanaceae, which includes species that produce a broad range of specialized metabolites. Here, we identified and analyzed the complement of genes in pepper () and potato (). We classified all genes into five groups based on their phylogenetic relationships and their gene and protein structures. Moreover, we discovered that tandem duplication contributed significantly to the expansion of the pepper family, while both tandem and segmental duplications contributed to the expansion of the potato family, indicating that took distinct evolutionary paths in these two Solanaceous species. Analysis of ω values showed that all potato and pepper genes experienced purifying selection during evolution. In addition, collinearity analysis showed that genes were highly conserved between pepper and potato. Analysis of -elements in promoters and expression patterns revealed that MATE proteins likely function in many stages of plant development, especially during fruit ripening, and when exposed to multiple stresses, consistent with the existence of functional differentiation between duplicated genes. Together, our results lay the foundation for further characterization of pepper and potato gene family members.
多药与有毒化合物外排（MATE）蛋白是一类重要的转运蛋白，可外排代谢产物，并参与植物发育和毒素解毒过程。对于茄科植物中的该基因家族，人们了解甚少，茄科包含能产生多种特殊代谢产物的物种。在此，我们鉴定并分析了辣椒（）和马铃薯（）中的基因互补情况。我们根据系统发育关系以及基因和蛋白质结构，将所有基因分为五组。此外，我们发现串联重复对辣椒基因家族的扩张有显著贡献，而串联重复和片段重复都对马铃薯基因家族的扩张有贡献，这表明在这两个茄科物种中，基因家族经历了不同的进化路径。ω值分析表明，所有马铃薯和辣椒基因在进化过程中都经历了纯化选择。此外，共线性分析表明，辣椒和马铃薯之间的基因高度保守。对基因启动子中的顺式作用元件和表达模式的分析表明，MATE蛋白可能在植物发育的许多阶段发挥作用，尤其是在果实成熟期间以及受到多种胁迫时，这与重复基因之间存在功能分化相一致。总之，我们的研究结果为进一步表征辣椒和马铃薯基因家族成员奠定了基础。
