Keya Jakia Jannat, Akter Mousumi, Yamasaki Yuta, Kageyama Yoshiyuki, Sada Kazuki, Kuzuya Akinori, Kakugo Akira
Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA.
Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48108, USA.
Small. 2025 Jul;21(26):e2408364. doi: 10.1002/smll.202408364. Epub 2024 Dec 23.
The assembly of biological systems forms nonequilibrium patterns with different functionalities through molecular-level communication via stepwise sequential interaction and activation. The mimicking of this molecular signaling offers extensive opportunities to design self-assemblies of bioinspired synthetic nonequilibrium systems to develop molecular robots with active, adaptive, and autonomous behavior. Herein, the design and construction of biomolecular motor system, microtubule (MT)-kinesin based molecular swarm system, are reported through stepwise sequential interactions of DNA. DNA signals are exchanged between three different DNA-tethered MTs, whereby the DNA signal from the first MT can activate the DNA strand on the second MT by communicating through physical contact, which facilitates assembly formation between the second and third DNA-tethered MTs. The DNA strands on the MTs can recognize the specific sequences of other DNA strands in the system and communicate with the complementary DNA on other MTs. This work will pave the way for developing autonomous molecular machines with advanced functionalities for complex nanotechnological applications.
生物系统的组装通过逐步顺序相互作用和激活进行分子水平的通信，形成具有不同功能的非平衡模式。对这种分子信号的模仿为设计受生物启发的合成非平衡系统的自组装提供了广泛的机会，以开发具有主动、自适应和自主行为的分子机器人。在此，通过DNA的逐步顺序相互作用，报道了基于微管（MT）-驱动蛋白的生物分子马达系统——分子群系统的设计与构建。DNA信号在三种不同的DNA连接的MT之间交换，由此来自第一个MT的DNA信号可以通过物理接触进行通信来激活第二个MT上的DNA链，这促进了第二个和第三个DNA连接的MT之间的组装形成。MT上的DNA链可以识别系统中其他DNA链的特定序列，并与其他MT上的互补DNA进行通信。这项工作将为开发具有先进功能的自主分子机器以用于复杂的纳米技术应用铺平道路。
