College of Food Science and Engineering, Jilin University, Changchun 130062, China.
College of Physics, Jilin University, Changchun 130062, China.
Int J Food Microbiol. 2022 Feb 2;362:109499. doi: 10.1016/j.ijfoodmicro.2021.109499. Epub 2021 Dec 9.
The study was to investigate the inhibitory effect and mechanism of high voltage prick electrostatic field (HVPEF) on Staphylococcus aureus biofilms. Results showed that HVPEF effectively inactivated 24-h and 48-h established S. aureus biofilms, and the effect was verified on different food-contact materials. Confocal laser scanning microscopy and scanning electron microscopy analysis suggested that HVPEF disintegrated the established biofilms by killing the embedded bacteria, but it hardly reduced the bacteria adhesion. HVPEF also effectively inhibit the formation of S. aureus biofilms, the effects varied with electric voltage, treatment time and biofilm culture conditions. The direct effect of HVPEF on planktonic S. aureus was a possible mode of biofilm formation inhibition. HVPEF also suppressed biofilm formation by reducing the release of key compositions of extracellular polymeric substance, including extracellular DNA (eDNA), protein and polysaccharide intercellular adhesion (PIA), and regulating the expression of biofilm formation related genes (icaA, ebh, cidA, sarA, icaR and sigB). We propose HVPEF as a novel method to inhibit bacteria biofilm, based on the results, HVPEF has positive effects to prevent biofilm-associated contamination of S. aureus.
该研究旨在探究高压电脉冲静电场（HVPEF）对金黄色葡萄球菌生物膜的抑制作用及其机制。结果表明，HVPEF 能有效杀灭 24 小时和 48 小时形成的金黄色葡萄球菌生物膜，且该效果在不同食品接触材料上均得到验证。共聚焦激光扫描显微镜和扫描电子显微镜分析表明，HVPEF 通过杀死嵌入的细菌来破坏已形成的生物膜，但对细菌黏附的减少作用较小。HVPEF 还能有效抑制金黄色葡萄球菌生物膜的形成，其效果随电压、处理时间和生物膜培养条件的不同而变化。HVPEF 对浮游态金黄色葡萄球菌的直接作用可能是其抑制生物膜形成的一种模式。HVPEF 通过减少细胞外聚合物（包括细胞外 DNA[eDNA]、蛋白质和胞间多糖黏附[PIA]）关键成分的释放，以及调节生物膜形成相关基因（icaA、ebh、cidA、sarA、icaR 和 sigB）的表达，来抑制生物膜的形成。基于这些结果，我们提出 HVPEF 是一种抑制细菌生物膜的新方法，它对预防金黄色葡萄球菌生物膜相关污染具有积极作用。
