Manuja Anju, Kumar Balvinder, Chhabra Dharvi, Brar Basanti, Riyesh T, Pal Yash, Bhattacharya T K, Prasad Minakshi
ICAR-National Research Centre On Equines, Hisar, Haryana 125001 India.
Lala Lajpat Rai, University of Veterinary and Animal Sciences, Hisar, India.
Indian J Microbiol. 2024 Sep;64(3):1123-1131. doi: 10.1007/s12088-024-01255-0. Epub 2024 Mar 30.
Ayurvedic medicine utilizes metal-based preparations, known as bhasmas, to treat various health conditions. Yasad bhasma (YB), a zinc-based ayurvedic preparation, shows promise as a potential candidate for developing zinc-based nanomedicines with anti-inflammatory and antioxidant properties. In this study, we synthesized a formulation combining YB and hydroxychloroquine (HC) as a zinc ionophore (YBHC) and investigated its biocompatibility and antiviral effects against buffalo calf coronavirus (BCoV) in Vero cells. Our results demonstrated that the formulation exhibited good conformity and enhanced cell proliferation compared to untreated cells. Additionally, no cytopathic effects were observed in BCoV-infected Vero cells treated with YBHC and YB, while infected control cells exhibited cytopathic effects. YB showed cytoprotection by promoting epithelial tissue turnover. We further explored whether YB/YBHC exerted a lysosomotropic effect to produce antiviral effects on coronavirus-adapted Vero cells, but no cell internalization was observed. In addition to the synergistic antiviral effect of YB and HC, YB may play a vital role in rejuvenating affected tissues.
阿育吠陀医学利用称为“bhasmas”的金属基制剂来治疗各种健康状况。雅沙德bhasma（YB），一种锌基阿育吠陀制剂，作为开发具有抗炎和抗氧化特性的锌基纳米药物的潜在候选物显示出前景。在本研究中，我们合成了一种将YB与作为锌离子载体的羟氯喹（HC）相结合的制剂（YBHC），并研究了其在Vero细胞中的生物相容性和对水牛犊冠状病毒（BCoV）的抗病毒作用。我们的结果表明，与未处理的细胞相比，该制剂表现出良好的一致性并增强了细胞增殖。此外，在用YBHC和YB处理的BCoV感染的Vero细胞中未观察到细胞病变效应，而感染的对照细胞表现出细胞病变效应。YB通过促进上皮组织更新显示出细胞保护作用。我们进一步探讨了YB/YBHC是否发挥溶酶体亲和效应以对冠状病毒适应的Vero细胞产生抗病毒作用，但未观察到细胞内化。除了YB和HC的协同抗病毒作用外，YB可能在使受影响的组织恢复活力方面发挥重要作用。
