多种反硝化菌中一氧化氮依赖的质子转运
Nitric oxide-dependent proton translocation in various denitrifiers.
作者信息
Shapleigh J P, Payne W J
出版信息
J Bacteriol. 1985 Sep;163(3):837-40. doi: 10.1128/jb.163.3.837-840.1985.
Abstract
Respiration of NO resulted in transient proton translocation in anaerobically grown cells of four physiologically diverse denitrifiers. Paracoccus denitrificans, Rhodopseudomonas sphaeroides subsp. denitrificans, "Achromobacter cycloclastes," and Rhizobium japonicum gave, respectively, H+/NO ratios of 3.65, 4.96, 1.94, and 1.12. Antimycin A completely inhibited NO-dependent proton translocation in P. denitrificans and severely restricted translocation in the R. sphaeroides strain. Proton uptake during NO respiration with antimycin A-inhibited cells supplied with an artificial electron source provided evidence for the periplasmic consumption of protons. Values obtained were consistent with the expected ratios of 0.5 mol of H+/mol of NO for reduction of NO to N2O and 1.0 mol of H+/mol of NO for reduction of NO to N2. These data are consistent with the presence of a unique NO reductase found only in anaerobically grown denitrifying cells.
摘要
在四种生理特性不同的反硝化细菌的厌氧生长细胞中，一氧化氮（NO）呼吸导致了瞬时质子转运。反硝化副球菌、脱氮红假单胞菌亚种、“环裂无色杆菌”和日本根瘤菌的H⁺/NO比率分别为3.65、4.96、1.94和1.12。抗霉素A完全抑制了反硝化副球菌中依赖NO的质子转运，并严重限制了球形红细菌菌株中的质子转运。在用抗霉素A处理的细胞中，当提供人工电子源时，NO呼吸过程中的质子摄取为周质质子消耗提供了证据。获得的值与将NO还原为N₂O时每摩尔NO消耗0.5摩尔H⁺以及将NO还原为N₂时每摩尔NO消耗1.0摩尔H⁺的预期比率一致。这些数据与仅在厌氧生长的反硝化细胞中发现的独特NO还原酶的存在相一致。
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本文引用的文献
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