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1
Nitric oxide-dependent proton translocation in various denitrifiers.多种反硝化菌中一氧化氮依赖的质子转运
J Bacteriol. 1985 Sep;163(3):837-40. doi: 10.1128/jb.163.3.837-840.1985.
2
Proton translocation and proline uptake associated with reduction of nitric oxide by denitrifying Paracoccus denitrificans.
Biochem Biophys Res Commun. 1982 Aug 31;107(4):1504-7. doi: 10.1016/s0006-291x(82)80169-1.
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Respiration-driven proton translocation with nitrite and nitrous oxide in Paracoccus denitrificans.反硝化副球菌中呼吸驱动的质子与亚硝酸盐和一氧化二氮的跨膜转运
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4
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Separate binding sites for antimycin and mucidin in the respiratory chain of the bacterium Paracoccus denitrificans and their occurrence in other denitrificans bacteria.反硝化副球菌呼吸链中抗霉素和杀稻瘟菌素的独立结合位点及其在其他反硝化细菌中的存在情况
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H218O isotope exchange studies on the mechanism of reduction of nitric oxide and nitrite to nitrous oxide by denitrifying bacteria. Evidence for an electrophilic nitrosyl during reduction of nitric oxide.利用H₂¹⁸O同位素交换研究反硝化细菌将一氧化氮和亚硝酸盐还原为一氧化二氮的机制。一氧化氮还原过程中亲电亚硝酰基的证据。
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8
The energy-conserving nitric-oxide-reductase system in Paracoccus denitrificans. Distinction from the nitrite reductase that catalyses synthesis of nitric oxide and evidence from trapping experiments for nitric oxide as a free intermediate during denitrification.反硝化副球菌中节约能量的一氧化氮还原酶系统。与催化一氧化氮合成的亚硝酸还原酶的区别以及反硝化过程中一氧化氮作为游离中间体的捕获实验证据。
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Biochemistry. 1978 Nov 14;17(23):5014-9. doi: 10.1021/bi00616a024.

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Spectroscopic characterization of heme iron-nitrosyl species and their role in NO reductase mechanisms in diiron proteins.血红素铁-亚硝酰基物种的光谱表征及其在双铁蛋白中一氧化氮还原酶机制中的作用。
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From no-confidence to nitric oxide acknowledgement: a story of bacterial nitric-oxide reductase.从不信任到一氧化氮认可:细菌一氧化氮还原酶的故事。
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本文引用的文献

1
Respiration-driven proton translocation with nitrite and nitrous oxide in Paracoccus denitrificans.反硝化副球菌中呼吸驱动的质子与亚硝酸盐和一氧化二氮的跨膜转运
Biochim Biophys Acta. 1981 Dec 14;638(2):181-91. doi: 10.1016/0005-2728(81)90226-7.
2
Proton translocation and proline uptake associated with reduction of nitric oxide by denitrifying Paracoccus denitrificans.
Biochem Biophys Res Commun. 1982 Aug 31;107(4):1504-7. doi: 10.1016/s0006-291x(82)80169-1.
3
Nitrogen oxide reduction in Wolinella succinogenes and Campylobacter species.琥珀酸沃林氏菌和弯曲杆菌属中氮氧化物的还原
J Bacteriol. 1982 Nov;152(2):915-8. doi: 10.1128/jb.152.2.915-918.1982.
4
Discrimination of ascorbate-dependent nonenzymatic and enzymatic, membrane-bound reduction of nitric oxide in denitrifying Pseudomonas perfectomarinus.脱氮完美海假单胞菌中抗坏血酸依赖性非酶促及酶促的膜结合一氧化氮还原的鉴别
Biochim Biophys Acta. 1982 Sep 15;681(3):459-68. doi: 10.1016/0005-2728(82)90188-8.
5
Proton translocation associated with nitrite respiration in Desulfovibrio desulfuricans.脱硫脱硫弧菌中与亚硝酸盐呼吸相关的质子转运
J Biol Chem. 1981 Jun 10;256(11):5450-8.
6
Stoichiometry of nitrite reduction catalyzed by Pseudomonas aeruginosa nitrite-reductase.铜绿假单胞菌亚硝酸还原酶催化的亚硝酸盐还原化学计量学。
Biochimie. 1984 Apr;66(4):313-8. doi: 10.1016/0300-9084(84)90008-7.
7
15N tracer studies on the reduction of nitrite by the purified dissimilatory nitrite reductase of Pseudomonas aeruginosa. Evidence for direct production of N2O without free NO as an intermediate.利用铜绿假单胞菌纯化的异化亚硝酸盐还原酶对亚硝酸盐还原进行的¹⁵N示踪研究。证明直接产生一氧化二氮,无游离一氧化氮作为中间产物。
J Biol Chem. 1983 Apr 25;258(8):4861-3.
8
Catalysis of nitrosyl transfer reactions by a dissimilatory nitrite reductase (cytochrome c,d1).异化亚硝酸盐还原酶(细胞色素c,d1)催化亚硝酰基转移反应。
J Biol Chem. 1984 Feb 25;259(4):2092-9.
9
The polarity of proton translocation in some photosynthetic microorganisms.一些光合微生物中质子转运的极性。
Eur J Biochem. 1969 Apr;8(3):450-4. doi: 10.1111/j.1432-1033.1969.tb00548.x.
10
Respiration-driven proton translocation in Micrococcus denitrificans.反硝化微球菌中呼吸驱动的质子转运
J Bioenerg. 1971 Sep;1(3):309-23. doi: 10.1007/BF01516290.

多种反硝化菌中一氧化氮依赖的质子转运

Nitric oxide-dependent proton translocation in various denitrifiers.

作者信息

Shapleigh J P, Payne W J

出版信息

J Bacteriol. 1985 Sep;163(3):837-40. doi: 10.1128/jb.163.3.837-840.1985.

DOI:10.1128/jb.163.3.837-840.1985
PMID:3928599
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC219207/
Abstract

Respiration of NO resulted in transient proton translocation in anaerobically grown cells of four physiologically diverse denitrifiers. Paracoccus denitrificans, Rhodopseudomonas sphaeroides subsp. denitrificans, "Achromobacter cycloclastes," and Rhizobium japonicum gave, respectively, H+/NO ratios of 3.65, 4.96, 1.94, and 1.12. Antimycin A completely inhibited NO-dependent proton translocation in P. denitrificans and severely restricted translocation in the R. sphaeroides strain. Proton uptake during NO respiration with antimycin A-inhibited cells supplied with an artificial electron source provided evidence for the periplasmic consumption of protons. Values obtained were consistent with the expected ratios of 0.5 mol of H+/mol of NO for reduction of NO to N2O and 1.0 mol of H+/mol of NO for reduction of NO to N2. These data are consistent with the presence of a unique NO reductase found only in anaerobically grown denitrifying cells.

摘要

在四种生理特性不同的反硝化细菌的厌氧生长细胞中,一氧化氮(NO)呼吸导致了瞬时质子转运。反硝化副球菌、脱氮红假单胞菌亚种、“环裂无色杆菌”和日本根瘤菌的H⁺/NO比率分别为3.65、4.96、1.94和1.12。抗霉素A完全抑制了反硝化副球菌中依赖NO的质子转运,并严重限制了球形红细菌菌株中的质子转运。在用抗霉素A处理的细胞中,当提供人工电子源时,NO呼吸过程中的质子摄取为周质质子消耗提供了证据。获得的值与将NO还原为N₂O时每摩尔NO消耗0.5摩尔H⁺以及将NO还原为N₂时每摩尔NO消耗1.0摩尔H⁺的预期比率一致。这些数据与仅在厌氧生长的反硝化细胞中发现的独特NO还原酶的存在相一致。