Parkkinen J, Finne J
J Biol Chem. 1985 Sep 15;260(20):10971-5.
The occurrence of phosphate-containing sialyl oligosaccharides in bovine colostrum was investigated. Two major sialyl oligosaccharide phosphates were identified, one of which was structurally similar to the previously characterized sialyl oligosaccharide 1-phosphates of human urine. The second sialyl oligosaccharide phosphate of bovine colostrum was found to be of a novel type. Structural studies including monosaccharide and phosphate analysis, glycosidase and phosphatase treatments, methylation analysis, and periodate treatment indicated the structure of this compound to be NeuAc alpha 2-6Gal beta 1-4GlcNAc-6-P. This provides the first evidence for the occurrence of N-acetylglucosamine 6-phosphate as an integral component in complex carbohydrates.
对牛初乳中含磷酸的唾液酸寡糖的存在情况进行了研究。鉴定出两种主要的唾液酸寡糖磷酸酯，其中一种在结构上与先前表征的人尿唾液酸寡糖1-磷酸酯相似。发现牛初乳中的第二种唾液酸寡糖磷酸酯是一种新型的。包括单糖和磷酸盐分析、糖苷酶和磷酸酶处理、甲基化分析以及高碘酸盐处理在内的结构研究表明，该化合物的结构为NeuAcα2-6Galβ1-4GlcNAc-6-P。这为N-乙酰葡糖胺6-磷酸作为复合碳水化合物的一个组成部分的存在提供了首个证据。
