Dezaki Farid Shirmardi, Narimani Tahmineh, Ghanadian Mustafa, Bidram Elham, Poursina Farkhondeh
Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Front Microbiol. 2024 Sep 2;15:1391039. doi: 10.3389/fmicb.2024.1391039. eCollection 2024.
One effective method to combat bacterial infections is by using bacteria itself as a weapon. is a type of fermenting bacterium that has probiotic properties and has demonstrated antimicrobial benefits against other bacteria. Cyclodipeptides (CDPs), present in the supernatant of , possess several antimicrobial properties.
In this study, the CDP fraction was isolated from the supernatant of (). This fraction was then loaded onto graphene oxide nanosheets (GO NSs). The study assessed the substance's ability to inhibit bacterial growth by using the minimum inhibitory concentration (MIC) method on and strains that were obtained from clinical samples. To determine the substance's impact on biofilm formation, the microtiter plate method was used. Moreover, the checkerboard technique was employed to explore the potential synergistic effects of these two substances.
According to the study, the minimum inhibitory concentration (MIC) of the desired compound was found to be 1.25 mg/mL against and 2.5 mg/mL against . Furthermore, at a concentration of 10 mg/mL, the compound prevented 81.6% ( < 0.01) of biofilm production in , while at a concentration of 1.25 mg/mL, it prevented 47.5% ( < 0.05) of biofilm production in . The study also explored the synergistic properties of two compounds using the checkerboard method.
In general, we found that GO NSs possess antimicrobial properties and enhance cyclodipeptides' activity against and .
对抗细菌感染的一种有效方法是将细菌本身用作武器。[具体细菌名称]是一种具有益生菌特性的发酵细菌，已证明对其他细菌具有抗菌益处。存在于[具体细菌名称]上清液中的环二肽（CDPs）具有多种抗菌特性。
在本研究中，从[具体细菌名称]（[具体菌株名称]）的上清液中分离出CDP组分。然后将该组分加载到氧化石墨烯纳米片（GO NSs）上。该研究通过对从临床样本中获得的[具体细菌名称1]和[具体细菌名称2]菌株使用最低抑菌浓度（MIC）方法，评估了该物质抑制细菌生长的能力。为了确定该物质对生物膜形成的影响，使用了微量滴定板法。此外，采用棋盘法来探索这两种物质的潜在协同作用。
根据该研究，发现所需化合物对[具体细菌名称1]的最低抑菌浓度（MIC）为1.25mg/mL，对[具体细菌名称2]为2.5mg/mL。此外，在浓度为10mg/mL时，该化合物在[具体细菌名称1]中阻止了81.6%（P<0.01）的生物膜产生，而在浓度为1.25mg/mL时，在[具体细菌名称2]中阻止了47.5%（P<0.05）的生物膜产生。该研究还使用棋盘法探索了两种化合物的协同特性。
总体而言，我们发现GO NSs具有抗菌特性，并增强了环二肽对[具体细菌名称1]和[具体细菌名称2]的活性。
